Two alternatively spliced transcripts generated from OsMUS81, a rice [Oryza sativa] homolog of yeast MUS81, are up-regulated by DNA-damaging treatments

• Published in: Plant and cell physiology Vol. 48; no. 4; pp. 648 - 654
• Main Authors: Mimida, N.(National Inst. of Agrobiological Sciences, Tsukuba, Ibaraki (Japan)), Kitamoto, H, Osakabe, K, Nakashima, M, Ito, Y, Heyer, W.D, Toki, S, Ichikawa, H
• Format: Journal Article
• Language: English
• Published: Japan Oxford University Press 01-04-2007; Oxford Publishing Limited (England)
• Subjects: ADN; Alternative Splicing - genetics; Alternative Splicing - radiation effects; Amino Acid Sequence; Base Sequence; DNA; DNA Damage - radiation effects; DNA, Plant; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - genetics; Endonucleases - chemistry; Endonucleases - genetics; Gene Expression Regulation, Plant - radiation effects; LEVADURA; LEVURE; Oryza - genetics; Oryza - radiation effects; ORYZA SATIVA; Plant Proteins - chemistry; Plant Proteins - genetics; PROTEINAS; PROTEINE; PROTEINS; REPAIRING; REPARACION; REPARATION; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Sequence Homology; Two-Hybrid System Techniques; Up-Regulation - genetics; Up-Regulation - radiation effects; YEASTS; Alternative splicing; XPF endonuclease superfamily; DNA repair; Homologous recombination; Rice
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcm029

OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.