One-step purification and characterization of an alkaline protease from haloalkaliphilic Bacillus sp

• Published in: Journal of Chromatography A Vol. 1075; no. 1; pp. 103 - 108
• Main Authors: Gupta, Anshu, Roy, Ipsita, Patel, R.K., Singh, S.P., Khare, S.K., Gupta, M.N.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20-05-2005; Elsevier
• Subjects: Alkaline protease; Bacillus - enzymology; Bacillus sp; Bacterial Proteins - antagonists & inhibitors; Bacterial Proteins - chemistry; Bacterial Proteins - isolation & purification; Bacteriology; Biological and medical sciences; Electrophoresis, Polyacrylamide Gel; Endopeptidases - chemistry; Endopeptidases - isolation & purification; Enzyme Inhibitors - pharmacology; Enzyme Stability; Fundamental and applied biological sciences. Psychology; Haloalkaliphilic; Hydrogen-Ion Concentration; Hydrophobic interaction chromatography; Metabolism. Enzymes; Microbiology; Protein purification; Surfactant stability; Temperature; Surfactant stability; Hydrophobic interaction chromatography; Haloalkaliphilic; Bacillus sp; Protein purification; Alkaline protease; Alkaline proteinase; Divalent metal Ions; Purification; Enzyme; Chemical stability; Bacillaceae; Surfactant; Thermal stability; Peptidases; Bacillales; Enzymatic activity; Salt effect; Hydrophobic chromatography; Medium effect; Bacillus; Bacteria; Hydrolases
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2005.03.127

An alkaline protease producer haloalkaliphilic bacteria (isolate Vel) was isolated from west coast of India. It was related to
Bacillus pseudofirmus on the basis of 16S r RNA gene sequencing, lipid profile and other biochemical properties. The protease secreted by this bacteria was purified 10-fold with 82% yield by a single step method on Phenyl Sepharose 6 Fast Flow column. The apparent molecular mass based on the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was estimated to be 29
000
Da. The
K
m and
V
max towards caseinolytic activity were found to be 2
mg
ml
−1 and 289.8
μg
min
−1, respectively. The enzyme was active over the pH range of 8.5–12.0, the optimum being 10–11.0. The purified enzyme when kept at 45
°C and 50
°C for 40
min retained 92% and 85% protease activity, respectively. Effect of NaCl concentration on protease activity showed that the enzyme was slightly inhibited with high concentration of salt. The proteolytic activity was inhibited by PMSF, suggesting that the enzyme may belong to serine type protease. Interestingly, the activity was slightly enhanced with SDS (0.1%) and Triton X-100 (0.1%) but remained unaffected by Tween 80 (0.1%). The activity was affected by metal ions to varying extent. While Mn
2+, Zn
2+ and Mg
2+ had no significant effect on protease activity, the enzyme was activated with Ca
2+ (1
mM) and Cu
2+ (5
mM). The stability of the enzyme in the presence of detergent components and surfactants is particularly attractive for its application in detergent industries.