Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons

• Published in: eLife Vol. 10
• Main Authors: Nguyen, Minh Q, von Buchholtz, Lars J, Reker, Ashlie N, Ryba, Nicholas Jp, Davidson, Steve
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 26-11-2021; eLife Sciences Publications, Ltd
• Subjects: Adult; Animals; Cell Nucleus - genetics; Dorsal root ganglia; Female; Ganglia, Spinal - metabolism; Gene Expression Profiling; Genes; Genomics; Humans; itch; Male; Mice; Middle Aged; Muscles; Neurons; Neurons - metabolism; Neuroscience; Pain; Proprioception; Single-Cell Analysis; somatosensation; Somatosensory system; Species; Tools and Resources; Transcriptome; Transcriptomics; Viscera; itch; mouse; pain; neuroscience; dorsal root ganglia; transcriptomics; somatosensation; human
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/elife.71752

Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to the skin, muscles, bones, and viscera to detect touch and temperature as well as to mediate proprioception and many types of interoception. In addition, the somatosensory system conveys the clinically relevant noxious sensations of pain and itch. Here, we used single nuclear transcriptomics to characterize transcriptomic classes of human DRG neurons that detect these diverse types of stimuli. Notably, multiple types of human DRG neurons have transcriptomic features that resemble their mouse counterparts although expression of genes considered important for sensory function often differed between species. More unexpectedly, we identified several transcriptomic classes with no clear equivalent in the other species. This dataset should serve as a valuable resource for the community, for example as means of focusing translational efforts on molecules with conserved expression across species.