Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes

Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by flowing fluids against the wall of vessels and epithelia...

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Bibliographic Details
Published in:	Bio-protocol Vol. 7; no. 8
Main Authors:	Shi, Shujie, Carattino, Marcelo D
Format:	Journal Article
Language:	English
Published:	United States Bio-Protocol 20-04-2017 Bio-protocol LLC
Subjects:	Biology Methods Xenopus oocytes Two-electrode voltage clamp Ion channels Microinjection Laminar shear stress Flow
Online Access:	Get full text
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Summary:	Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by flowing fluids against the wall of vessels and epithelia. The protocol outlined herein has been used to examine the response of ion channels expressed in oocytes to LSS (Hoger , 2002; Carattino , 2004; Shi , 2006). The oocyte is a reliable system that allows for the expression and chemical modification of ion channels and regulatory proteins (George , 1989; Palmer , 1990; Sheng , 2001; Carattino , 2003). Therefore, this technique is suitable for studying the molecular mechanisms that allow flow-activated channels to respond to LSS.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2331-8325 2331-8325
DOI:	10.21769/bioprotoc.2224