Using Hypercrosslinked Polystyrene for the Multicomponent Solid-Phase Extraction of Residues of 63 Veterinary Preparations in Their Determination in Chicken Meat by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 76; no. 8; pp. 946 - 959
• Main Authors: Melekhin, A. O., Tolmacheva, V. V., Shubina, E. G., Dmitrienko, S. G., Apyari, V. V., Grudev, A. I.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-08-2021; Springer; Springer Nature B.V
• Subjects: Analysis; Analytical Chemistry; Antibiotics; Buffer solutions; Cartridges; Chemistry; Chemistry and Materials Science; Chickens; Contamination; Demeclocycline; Ethylenediaminetetraacetic acid; Hexanes; High performance liquid chromatography; Ions; Mass spectrometry; Meat; Polystyrene; Polystyrene resins; Residues; Scientific imaging; Solid phases; Spectroscopy; Sulfonamides; Tetracyclines; Russia; multicomponent solid-phase extraction; high-performance liquid chromatography; hypercrosslinked polystyrene; tandem mass spectrometry; veterinary preparations; chicken meat
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934821060046

Hypercrosslinked polystyrene is proposed for the multicomponent solid-phase extraction of residues of 63 veterinary preparations from various classes (sulfonamides, tetracyclines, quinolones, amphenicols, nitroimidazoles, β-lactams, macrolides, lincosamides, and pleuromutilins) in their determination in chicken meat by HPLC–tandem mass spectrometry. Sample preparation includes the extraction of analytes with a McIlvaine buffer solution, fat removal by extraction with hexane, and the further purification of the extracts on cartridges with hypercrosslinked polystyrene. The method ensures the quantitative extraction of analytes (recovery rates are from 83 to 117%) and good reproducibility (RSD ≤ 12%). Veterinary preparations were identified by the exact masses of analyte ions formed in electrospray ionization on switching positive and negative polarity. The matrix effect for all veterinary drugs was below 20%. The determination was carried out by the method of matrix calibration; the limits of detection and determination were 0.01–0.3 and 0.02–1 ng/g, respectively. An analysis of contaminated chicken meat samples showed that the results are in satisfactory agreement with the data obtained by the methods adopted in the Russian Federation for the determination of various groups of antibiotics.