Handheld line-scanned dual-axis confocal microscope with pistoned MEMS actuation for flat-field fluorescence imaging

A handheld line-scanned dual-axis confocal (LS-DAC) microscope has been developed for high-speed (16 frames/s) fluorescence imaging of tissues with sub-nuclear resolution. This is the first miniature fluorescence LS-DAC system that has been fully packaged for handheld clinical use on patients. A nov...

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Bibliographic Details
Published in:	Optics letters Vol. 44; no. 3; p. 671
Main Authors:	Wei, Linpeng, Yin, Chengbo, Fujita, Yoko, Sanai, Nader, Liu, Jonathan T C
Format:	Journal Article
Language:	English
Published:	United States 01-02-2019
Subjects:	Animals Equipment Design Image Processing, Computer-Assisted Kidney - diagnostic imaging Mice Micro-Electrical-Mechanical Systems Microscopy, Confocal - instrumentation Optical Imaging - instrumentation
Online Access:	Get more information
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Summary:	A handheld line-scanned dual-axis confocal (LS-DAC) microscope has been developed for high-speed (16 frames/s) fluorescence imaging of tissues with sub-nuclear resolution. This is the first miniature fluorescence LS-DAC system that has been fully packaged for handheld clinical use on patients. A novel micro-electro-mechanical system scanning mechanism, with synchronized tilting and pistoning, is used to achieve flat-field en face imaging. We show that this facilitates video mosaicking to generate images that sample an extended lateral field of view.
ISSN:	1539-4794
DOI:	10.1364/OL.44.000671