Analysis of ACE inhibitors in pharmaceutical dosage forms by derivative UV spectroscopy and liquid chromatography (HPLC)

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 16; no. 3; pp. 431 - 438
• Main Authors: Bonazzi, D, Gotti, R, Andrisano, V, Cavrini, V
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-11-1997; Elsevier Science
• Subjects: ACE inhibitors; Analysis; Angiotensin-Converting Enzyme Inhibitors - analysis; Biological and medical sciences; Calibration; Capsules; Chromatography, High Pressure Liquid; Derivative spectroscopy; Drug analysis; General pharmacology; High performance liquid chromatography; Hydrogen-Ion Concentration; Medical sciences; Pharmaceutical dosage forms; Pharmacology. Drug treatments; Spectrophotometry, Ultraviolet; Tablets; Pharmaceutical dosage forms; Drug analysis; Derivative spectroscopy; ACE inhibitors; High performance liquid chromatography; Enzyme; HPLC chromatography; Enzyme inhibitor; Peptidases; Separation method; Reversed phase chromatography; Ultraviolet detector; Peptidyl-dipeptidase A; Quality control; Dosage form; Hydrolases; Peptidyl-dipeptidases; Spectrophotometry; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(97)00075-7

Derivative UV spectroscopy and high performance liquid chromatography (HPLC) were applied to the determination of angiotensin-converting enzyme (ACE) inhibitors in their pharmaceutical dosage forms. For spectrophotometric determinations, the more appropriate derivative order was selected for each drug: ramipril (third-order), benazepril (second-order), enalapril maleate (second-order), lisinopril (first- and second-order) and quinapril (first-order). Reverse phase HPLC procedures (ODS column) were developed able to provide a single, symmetric peak for each drug; mixtures A–B, where A is 20 mM sodium heptansulphonate (pH 2.5) and B is acetonitrile–THF (95:5 v/v), proved to be suitable mobile phases to obtain selective separations of the cited ACE inhibitors. At ambient temperature, a low pH value (2.5) was found to be critical to avoid peak splitting and band broadening.