Lorazepam induces acinar cells apoptosis of rat parotid glands

• Published in: The Saudi dental journal Vol. 32; no. 6; pp. 276 - 282
• Main Authors: Bettega, Patrícia Vida Cassi, Johann, Aline Cristina Batista Rodrigues, Rinaldi, Mariana, Ignácio, Sérgio Aparecido, Rosa, Edvaldo Antônio Ribeiro, Alanis, Luciana Reis Azevedo, Althobaiti, Yusuf, Sapelli, Silvana da Silva, Hardy, Ana Maria Trindade Grégio
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-09-2020; Elsevier
• Subjects: Acinar cells; Anxiety; Apoptosis; Depression; Lorazepam; Original; Salivary glands; Acinar cells; Lorazepam; Depression; Anxiety; Salivary glands; Apoptosis
• ISSN: 1013-9052; 1658-3558
• DOI: 10.1016/j.sdentj.2019.09.006

To evaluate the apoptotic action of Lorazepam on acinar cells of the parotid glands of rats, forty male Wistar rats were separated into four groups. Saline was given to the control groups for thirty (GS30) and sixty days (GS60). L30 received Lorazepam for thirty days and GL30 + S30 Lorazepam for thirty days, and from the thirty-first day, saline. For the counting of apoptotic cell nuclei, TUNEL technique was applied. The statistical tests used were ANOVA and Tukey HSD. On the thirtieth day, there was no statistically significant difference between GL30 and GS30, in relation to the number of apoptotic cell nuclei and cell proliferation. There was a statistically significant difference in the number of apoptotic cell nuclei in GL30 compared to GS30. The percentage of apoptotic cell nuclei from the GL30+S30 was significantly higher in relation to GS60. A statistically significant reduction in the value of acinar cell nuclei was also observed between these groups. No significant difference was found in the values of cell proliferation among the GL30+S30 and GS60 groups. Lorazepam caused significant decrease in the number of acinar cell nuclei and larger percentage of cellular apoptotic nuclei in rat parotid glands.