In vitro gossypol induced spermatozoa motility alterations in rabbits

• Published in: Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Vol. 44; no. 7; pp. 730 - 741
• Main Authors: Roychoudhury, Shubhadeep, Massanyi, Peter, Slamecka, Jaroslav, Chlebec, Ivan, Trandzik, Jozef, Bulla, Jozef, Okab, Aly B, Taha, Taha A, Salem, Mohamed H, Ayoub, Mostafa A
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 01-09-2009; Taylor & Francis
• Subjects: Agronomy. Soil science and plant productions; Animals; Biological and medical sciences; chemical constituents of plants; Computer Assisted Semen Analyzer; cottonseed meal; Cottonseed Oil - chemistry; Cottonseed Oil - toxicity; dose response; feed supplements; Fundamental and applied biological sciences. Psychology; gossypol; Gossypol - toxicity; in vitro studies; Infertility, Male - chemically induced; Male; rabbit; Rabbits; semen; Soil and water pollution; Soil science; sperm motility; Sperm Motility - drug effects; spermatozoa; Spermatozoa - drug effects; Spermatozoa motility; toxicity testing; Spermatozoa; Motility; Semen; Computer Assisted Semen Analyzer; Alteration; Rabbit; Lagomorpha; Analyzer; Gossypol; In vitro; Vertebrata; Mammalia; Environment; Computer aid; Spermatozoa motility
• ISSN: 0360-1234; 1532-4109
• DOI: 10.1080/03601230903163905

The objectives of the present study were to: (i) examine the in vitro dose response of rabbit spermatozoa motility to the antifertility agent gossypol (GOS) and (ii) determine whether filtered (FIL) and unfiltered (UNFIL) GOS differ in their magnitude of effect. Rabbit semen belonging to adult males (n = 5; 12-14 months) were cultured with UNFIL GOS and FIL GOS (5% solution) and subsequently diluted (1:1-7) for analysis using a Computer Assisted Semen Analyzer (CASA) system in 5 time periods (0, 60, 120, 180 and 360 minutes). At Time 0, no significant change in rabbit spermatozoa motility (MOT) and progressive motility (PROG) with GOS FIL was noted, while increases were observed with GOS UNFIL. At Time 60, weak changes were noted for MOT and PROG. After 120 minutes of culture with both GOS FIL and GOS UNFIL, MOT and PROG decreased significantly in some experimental groups. However, no differences were recorded for both the parameters at Times 180 and 360, with the exception of PROG in the GOS UNFIL category (groups A, B, E, F and G), where a significant decrease was noticed. Detailed evaluation of the distance and velocity parameters revealed reduction in all these studied markers after 60 and 120 minutes of in vitro culture with both GOS FIL and GOS UNFIL, indirectly confirming the PROG decrease. Straightness (STR), linearity (LIN), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) mostly remained unaltered at all time periods for GOS FIL, where as some minor alterations were noticed in GOS UNFIL category for STR, LIN, WOB, ALH and BCF parameters at Time 0, 60 and 120. The present study confirms the dose and time dependent alterations of rabbit spermatozoa motility parameters by GOS. The GOS dynamics in our experiment shows that rabbit spermatozoa as a biological material can indicate a GOS inhibition of motility. Obtained data for the first time indicates a higher immobilizing potential of unfiltered GOS in comparison to filtered GOS in its inhibitory action of spermatozoa motility parameters in rabbits.