Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children

• Published in: Oral surgery, oral medicine, oral pathology and oral radiology Vol. 113; no. 3; pp. 378 - 383
• Main Authors: Portela, Maristela Barbosa, das Chagas, Madeleine Souza, Cerqueira, Daniella Ferreira, de Souza, Ivete Pomarico Ribeiro, Souto-Padrón, Thaís, de Araújo Soares, Rosangela Maria, de Araújo Castro, Gloria Fernanda Barbosa
• Format: Journal Article
• Language: English
• Published: United States Mosby, Inc 01-03-2012
• Subjects: Candida albicans - enzymology; Child; Child, Preschool; Collagen Type I - metabolism; Dental Caries - complications; Dental Caries - microbiology; Dentin - microbiology; Dentistry; Female; HIV Infections - complications; Humans; Male; Mouth Mucosa - microbiology; Proteolysis; Statistics, Nonparametric; Surgery
• ISSN: 2212-4403; 2212-4411
• DOI: 10.1016/j.oooo.2011.09.006

Objective The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children. Study Design To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120. Results Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P < .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P > .05). Conclusions Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I collagen.