Quantification of glycosidase activities in selected yeasts and lactic acid bacteria

• Published in: Journal of industrial microbiology & biotechnology Vol. 23; no. 3; pp. 198 - 203
• Main Authors: McMahon, H, Zoecklein, B.W, Fugelsang, K, Jasinski, Y
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-09-1999; Oxford University Press
• Subjects: a-Arabinofuranosidase; a-Rhamnopyranosidase; Acids; Alcohol; Aureobasidium; Bacteria; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Candida; Catalysis; Cryptococcus; enological yeasts; Enzymatic activity; Enzymes; Fermentation; Fermented food industries; flavor compounds; Food industries; Food science; Fundamental and applied biological sciences. Psychology; Glucose; glycosidase; grapes; Leuconostoc; Leuconostoc oenos; Microbiology; Miscellaneous; Mission oriented research; Oenococcus oeni; Phenols; Saccharomyces; Studies; Wineries & vineyards; wines; Wines and vinegars; Yeast; Yeasts; Wine; Lactic acid bacteria; Yeast; Enzyme; α-L-Arabinofuranosidase; Fungi; Hydrolysis; Brettanomyces; β-Glucosidase; Enzymatic activity; Glycosidases; Grape; Bacteria; Hydrolases; Ascomycetes; Glycoside; Fungi Imperfecti; Aroma; Oenococcus oeni; O-Glycosidases; Saccharomyces cerevisiae; Thallophyta
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1038/sj.jim.2900720

Using a model system, the activities of alpha-L-arabinofuranosidase, beta-glucosidase, and alpha-L-rhamonopyranosidase were determined in 32 strains of yeasts belonging to the genera Aureobasidium, Candida, Cryptococcus, Hanseniaspora, Hansenula, Kloeckera, Metschnikowia, Pichia, Saccharomyces, Torulaspora and Brettanomyces (10 strains); and seven strains of the bacterium Leuconostoc oenos. Only one Saccharomyces strain exhibited beta-glucosidase activity, but several non-Saccharomyces yeast species showed activity of this enzyme. Aureobasidium pullulans hydrolyzed alpha-L-arabinofuranoside, beta-glucoside, and alpha-L-rhamnopyranoside. Eight Brettanomyces strains had beta-glucosidase activity. Location of enzyme activity was determined for those species with enzymatic activity. The majority of glucosidase activity was located in the whole cell fraction, with smaller amounts found in permeabilized cells and released into the growth medium. Aureobasidium pullulans hydrolyzed glycosides found in grapes.