Chronic REM sleep restriction in young rats increases energy expenditure with no change in food intake

• Published in: Experimental physiology Vol. 105; no. 8; pp. 1339 - 1348
• Main Authors: Menezes, Letícia, Moraes, Danilo Alves, Ribeiro‐Silva, Neila, Silva, Sylvia Maria Affonso, Suchecki, Deborah, Luz, Jacqueline
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01-08-2020
• Subjects: Animal behavior; Body fat; Body weight gain; Body weight loss; Dietary intake; Energy; Energy balance; Energy expenditure; Food; Food intake; Metabolism; REM sleep; REM sleep restriction; Rodents; Sleep; Sleep deprivation; energy balance; metabolism; REM sleep restriction
• ISSN: 0958-0670; 1469-445X
• DOI: 10.1113/EP088474

New Findings
What is the central question of this study?
What are the effects of different periods of REM sleep restriction (7, 14 and 21 days) on metabolic parameters in young rats?
What is the main finding and its importance?
Animals submitted to each period of REM sleep deprivation showed a negative energy balance, with reduced body weight gain, body energy gain and gross food efficiency, less body fat content, and increased energy expenditure. There was no increase in food intake after any of the REM sleep restriction periods. In young rats, negative energy balance is not compensated by increased dietary intake as observed in adult rats.
Reduced sleep is associated with metabolic alterations, not only in adults, but also in children and adolescents. Several studies have shown that sleep restricted (SR) adult rats exhibit metabolic changes, followed by increased food intake, but few have evaluated these functions in young animals. The aim of the present study was to establish the metabolic parameters of young rats subjected to different periods of REM sleep restriction and to propose a correction factor for the correct measurement of food intake. Young male Wistar rats were distributed in control and SR groups for 7, 14 or 21 days. Sleep restriction was performed by the single platform method for 18 h. Regardless of the length of sleep restriction, all SR rats had a negative energy balance, evidenced by reduction in body weight gain, body energy gain and gross food efficiency, accompanied by increased energy expenditure. In addition, sleep restriction reduced body fat content throughout the entire period. Discounting food spillage, there was no increase in food intake by SR rats. In conclusion, the present study revealed metabolic changes in SR young rats after different lengths of REM sleep restriction and that weight loss and increased energy expenditure were not compensated by increased dietary intake as occurs in adult rats, indicating that young rats use other mechanisms to cope with the negative energy balance caused by sleep restriction. In addition, we propose a correction factor for food intake, to prevent overestimation of this parameter, due to food spillage in the water containers.