LncRNA LINC01871 sponging miR-142-3p to modulate ZYG11B promotes the chemoresistance of colorectal cancer cells by inducing autophagy

• Published in: Anti-cancer drugs Vol. 34; no. 7; pp. 827 - 836
• Main Authors: Duan, Bensong, Zhang, Haibin, Zhu, Zhen, Yan, Xiaohan, Ji, Zhonghua, Li, Jingze
• Format: Journal Article
• Language: English
• Published: England The Author(s). Published by Wolters Kluwer Health, Inc 01-08-2023; Lippincott Williams & Wilkins
• Subjects: Autophagy; Cell Line, Tumor; Cell Proliferation - genetics; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - genetics; Drug Resistance, Neoplasm - genetics; Humans; MicroRNAs - genetics; MicroRNAs - metabolism; Pre-Clinical Reports; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism
• ISSN: 0959-4973; 1473-5741
• DOI: 10.1097/CAD.0000000000001478

Colorectal cancer (CRC) is a malignant tumor in the digestive tract. Increasing evidence indicated that chemoresistance leads to a poor prognosis of CRC. Herein, we aimed to uncover the potential mechanism by which long intergenic noncoding RNA-1871 (LINC01871) affects the chemoresistance of CRC cells.
Relative level of LINC01871 in CRC tissues was assessed by reverse transcription quantitative PCR (RT-qPCR). Kaplan-Meier analysis was conducted to determine the relevance of LINC01871 and the prognosis of CRC patients. Cell Counting Kit-8 (CCK-8) and colony formation assay were used to evaluate the proliferation of SW480 cells. Expression levels of proteins and their genes were assessed by western blot, immunofluorescence staining and RT-qPCR. In addition, the interaction of LINC01871, miR-142-3p and protein zyg-11 homolog B (ZYG11B) were analyzed via dual-luciferase reporter assays.
LINC01871 was low-expressed in CRC tissues and cell lines. Patients with a low level of LINC01871 showed significantly lower survival rate. pcDNA-LINC01871 significantly reduced the viability of SW480 cells ( P  < 0.01), elevated SW480 cells sensitivity to 5-FU ( P  < 0.01), reduced LC3 punctate aggregates ( P  < 0.01) and downregulated the relative mRNA expression level of autophagy related protein 9A, autophagy related protein 4B and high mobility group box 1 ( P  < 0.01) in SW480 cells. Moreover, LINC01871 was found to sponge miR-142-3p, and ZYG11B was the target of miR-142-3p. MiR-142-3p mimic significantly recovered the effect of pcDNA-LINC001871, whereas pcDNA-ZYG11B reversed the recovery effect of the miR-142-3p mimic.
LINC01871/miR-142-3p/ ZYG11B axis regulates the chemoresistance of CRCs by inducing autophagy.