PFOS and PCB 153 have direct adverse effects on neonatal testis modeled using a coculture of primary gonocyte and sertoli cells

• Published in: Environmental toxicology Vol. 28; no. 6; pp. 322 - 331
• Main Authors: Zhang, Jie, Liang, Jiren, Zhu, Hongyan, Li, Cuizhen, Wu, Qing
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-06-2013; Wiley; Wiley Subscription Services, Inc
• Subjects: Alkanesulfonic Acids - toxicity; Animal, plant and microbial ecology; Animals; Animals, Newborn; apoptosis; Apoptosis - drug effects; Applied ecology; Biological and medical sciences; Cell Survival - drug effects; coculture; Coculture Techniques; cytoskeleton; Cytoskeleton - drug effects; Cytoskeleton - ultrastructure; Ecotoxicology, biological effects of pollution; Environmental Pollutants - toxicity; exposure; Fluorocarbons - toxicity; Fundamental and applied biological sciences. Psychology; General aspects; Germ Cells - cytology; Germ Cells - drug effects; gonocyte; Male; neonatal; PCB; PCB 153; PFOS; Polychlorinated biphenyls; Polychlorinated Biphenyls - toxicity; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Rodents; ROS; Sertoli; Sertoli Cells - cytology; Sertoli Cells - drug effects; Sertoli cell; Sertoli; Ecotoxicology; Toxicity; PCB 153; Polychlorobiphenyls; neonatal; Testicle; coculture; exposure; ROS; Cytoskeleton; Environment; Models; Fluorine Organic compounds; gonocyte; Apoptosis; PFOS
• ISSN: 1520-4081; 1522-7278
• DOI: 10.1002/tox.20723

Perfluorooctane sulfonate (PFOS) is widely used in industry; it is nonbiodegradable and persistent in the human body and in the environment. Although reports have indicated that young people might have higher PFOS levels in serum or blood than do older people, its adverse effects on neonatal testicular cells had not been investigated previously. PCB 153 is one of the most prevalent polychlorinated biphenyl (PCB) congeners in biological tissues, but the direct adverse effect of PCB 153 on neonatal testis remains unclear. In this study, we exposed a neonatal Sertoli cell/gonocyte coculture system to PFOS and PCB 153 individually at concentrations of 0, 1, 10, 50, and 100 μM for 24 h. Exposure to either compound reduced the cell viability and induced the production of reactive oxygen species (ROS) in dose‐dependent manners, with PCB 153 having a greater effect than PFOS. Whereas PCB 153 induced apoptosis significantly from 10 μM, PFOS induced no obvious change. Morphologically, both PCB 153 and PFOS induced changes in the vimentin and actin filaments in the Sertoli cells, as investigated using confocal argon ion laser scanning microscopy; here, PFOS exhibited a more dramatic effect than did PCB 153. Furthermore, doses of 50 μM for PFOS and 10 μM for PCB 153 were the key concentrations that produced significant differences between the control and exposure groups. We suggest that both PCB 153 and PFOS directly affect neonatal gonocyte and Sertoli cells; the production of ROS and the change in the cytoskeleton in Sertoli cells might be causes. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2013.