Application of the CRISPR-Cas System for Efficient Genome Engineering in Plants

• Published in: Molecular plant Vol. 6; no. 6; pp. 2008 - 2011
• Main Authors: Mao, Yanfei, Zhang, Hui, Xu, Nanfei, Zhang, Botao, Gou, Feng, Zhu, Jian-Kang
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01-11-2013; Cell Press; Oxford University Press
• Subjects: Arabidopsis - genetics; CAS系统; Clustered Regularly Interspaced Short Palindromic Repeats; DNA双链断裂; Genetic Engineering; Genome, Plant; Letter to the Editor; Oryza - genetics; Plants, Genetically Modified; 同源重组; 基因组工程; 应用; 核酸内切酶; 植物基因; 重复序列
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1093/mp/sst121

Dear Editor, Recently, engineered endonucleases, such as Zinc-Finger Nucleases （ZFNs） （Carroll, 2011）, Transcription Activator-Like Effector Nucleases （TALENs） （Mahfouz et al., 2011; Li et al., 2012）, and Clustered Regularly Interspaced Short Palindromic Repeats （CRISPR）-associated （Cas） systems （Cong et al., 2013） have been successfully used for gene editing in a variety of species. These systems generate double-strand breaks （DSBs） at target loci to drive site-specific DNA sequence modifica- tions. The modifications include sequence insertion and deletion and other mutations in the host genomes via the error-prone non-homologous end joining （NHEJ） pathway or sequence correction or replacement through the error-free homologous recombination （HR） pathway （Symington and Gautier, 2011）. Here, we show that the CRISPR-Cas system can be applied to generate targeted gene mutations and gene corrections in plants, and the system can also be readily engi- neered to achieve deletion of large DNA fragments and for multiplex gene editing in plants.