Detection of Epstein-Barr virus (EBV) in the oral mucosa of renal transplant patients

• Published in: Diagnostic cytopathology Vol. 34; no. 1; pp. 24 - 28
• Main Authors: Braz-Silva, Paulo H., Ortega, Karem L., Rezende, Nathalie P., Nunes, Fabio D., Magalhães, Marina H.C.G.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-01-2006
• Subjects: DNA, Viral - analysis; EBV-DNA; Epstein-Barr virus; Gingiva - virology; Herpesvirus 4, Human - drug effects; Herpesvirus 4, Human - isolation & purification; Humans; Immunocompromised Host; immunocompromised patients; Kidney Transplantation - adverse effects; Mouth Mucosa - virology; Mouthwashes - therapeutic use; oral brush cytology; PBS rinse; PCR; Polymerase Chain Reaction; Retrospective Studies; Saliva - virology; Sodium Chloride - pharmacology; Tongue - virology
• ISSN: 8755-1039; 1097-0339
• DOI: 10.1002/dc.20380

The purpose of this study was to determine the prevalence of Epstein‐Barr virus (EBV)‐DNA in the oral mucosa of renal transplant patients and observe the efficacy of mouth rinses with phosphate‐buffered saline (PBS) to eliminate EBV present in the saliva. Lingual, gingival, and buccal cytobrushings were obtained from normal oral mucosa of 10 renal transplant patients and 10 normal subjects, and were examined through polymerase chain reaction (PCR), before and after rinses with PBS. EBV‐DNA was detected in 86.6% of renal transplant recipients and in 46.6% of healthy subjects. No significant difference was observed between oral scrapes obtained before and after rinses with PBS with regard to detection of EBV‐DNA. Our results suggest that the use of PCR to detect the presence of EBV‐DNA in oral mucosa in the absence of specific lesions gives rise to the problem of identifying the viral replication sites. In addition, PBS was not effective at minimizing contamination by saliva. Diagn. Cytopathol. 2006;34:24–28. © 2005 Wiley‐Liss, Inc.