Activities of enzymes that hydrolyze adenine nucleotides in lymphocytes from patients with rheumatoid arthritis

• Published in: Cell biochemistry and function Vol. 31; no. 5; pp. 395 - 399
• Main Authors: dos Santos Jaques, Jeandre Augusto, Becker, Lara Vargas, Souza, Viviane do Carmo Gonçalves, Leal, Cláudio Alberto Martins, Bertoldo, Tatiana Montagner Dalcin, de Vargas Pinheiro, Kelly, Morsch, Vera Maria, Schetinger, Maria Rosa Chitolina, Leal, Daniela Bitencourt Rosa
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01-07-2013; Wiley Subscription Services, Inc
• Subjects: Adenosine Deaminase - metabolism; Adenosine Diphosphate - metabolism; Adenosine Triphosphate - metabolism; Adult; Antigens, CD - metabolism; Apyrase - metabolism; Arthritis, Rheumatoid - enzymology; Arthritis, Rheumatoid - pathology; Case-Control Studies; Cells, Cultured; E-ADA; E-NTPDase; Enzyme Assays; Female; Humans; lymphocytes; Lymphocytes - enzymology; Lymphocytes - pathology; Male; Middle Aged; nucleotides; rheumatoid arthritis; E-NTPDase; lymphocytes; nucleotides; rheumatoid arthritis; E-ADA
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.2910

The purpose of this study was to investigate the activities of ecto‐nucleoside triphosphate diphosphohydrolase (E‐NTPDase; EC 3.6.1.5; CD39) and adenosine deaminase (E‐ADA; EC 3.5.4.4) in lymphocytes from patients with rheumatoid arthritis (RA). Thirty patients diagnosed with RA through American College of Rheumatology criteria as well as 30 healthy patients were selected. Peripheral blood lymphocytes were isolated, and E‐NTPDase and E‐ADA activities were assayed. The results demonstrated an increased E‐NTPDase activity (both ATP and ADP as substrates) and a decreased E‐ADA activity in RA patients. These data suggest an organic effort to preserve the adenosine level, which is known to have anti‐inflammatory and analgesic properties, working as a potent suppressor of immune response. Copyright © 2012 John Wiley & Sons, Ltd.