The Effect of Static Magnetic Fields of Different Strengths and Polarities on Cytokine Production by Human Lymphocytes In Vitro

• Published in: Bioengineering (Basel) Vol. 11; no. 8; p. 749
• Main Authors: Turuntaš, Vladimir, de Luka, Silvio, Ristić-Djurovic, Jasna L, Ćirković, Saša, Djordjevich, Drago, Ristić, Siniša, Lalović, Nenad, Marić, Veljko, Lazić, Bratislav, Joksimović, Bojan, Stanojevic, Ivan, Vasilijić, Saša, Trbovich, Alexander M
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24-07-2024; MDPI
• Subjects: Antigen-presenting cells; Apoptosis; B cells; Biological effects; Cell culture; Cytokines; Dendritic cells; Electromagnetic fields; Enzyme-linked immunosorbent assay; Exposure; Flow cytometry; IL-1β; Immune response; Immune system; Intellectual property; Interleukin 1; Interleukin 10; Interleukin 17; Interleukin 4; Interleukin 6; Interleukin 8; Lymphocytes; Lymphocytes T; Magnetic fields; Magnetic poles; Magnetic resonance imaging; NMR; Nuclear magnetic resonance; static magnetic field; T cells; TLR3 protein; TLR7 protein; Toll-like receptors; Tumor necrosis factor; Tumor necrosis factor-α; γ-Interferon; Serbia; United Kingdom; Germany; Costa Mesa California; Minneapolis Minnesota; Austria; United Kingdom > UK; United States > US; Vienna Austria; cytokines; lymphocytes; static magnetic field
• ISSN: 2306-5354; 2306-5354
• DOI: 10.3390/bioengineering11080749

In contrast to electromagnetic fields, static magnetic fields (SMFs) have not been extensively studied in terms of their potential health consequences. Although upward- and downward-oriented magnetic poles may cause various biological effects, only the pole with the upward orientation has been mainly investigated. Considering that the interaction of antigen-presenting cells (APCs) and T lymphocytes is crucial to trigger an immune response, we assessed the effect of long-term exposure of human T lymphocytes and dendritic cells (DCs) to moderate strength SMFs of different orientations focusing on the cytokine profile of activated T cells. Cultures of allogenic T lymphocytes and DCs (immature and matured by TLR3 and TLR7 agonists) were continuously exposed to four SMFs. The intensity of the applied field was 1 militesla (mT) or 56 mT of the upward- and downward-oriented pole of the SMF. Cell culture supernatants were assayed for IFN-γ, IL-4, IL-17, TNF-α, TNF-β, IL-1 β, IL-6, IL-8, and IL-10 by ELISA or flow cytometry. The upward-oriented 56 mT SMF significantly increased the release of IFN-γ and TNF-β (both
< 0.05) in the cell culture supernatants of T cells and immature DCs. In contrast, the same cultures exposed to the upward-oriented 1 mT SMF showed significantly elevated levels of IL-17 (
< 0.05). The levels of IL-4, TNF-α, IL-1 β, IL-6, IL-8, and IL-10 were not affected by the upward-oriented SMF. The downward-oriented 56 mT SMF increased TNF-α release when T cells were stimulated with mature DCs. The production of other cytokines was unchanged by the downward-oriented SMF. These findings demonstrate for the first time different in vitro biological effects of upward- and downward-oriented static magnetic fields on the cytokine production of T cells activated by DCs, helping to better understand SMF effects on the immune system and suggesting that the selective SMF effect on the immune response could have potential therapeutic effects in different immune-mediated disorders.