Comparison of Surface Modification Techniques on Polydimethylsiloxane to Prevent Protein Adsorption

• Published in: Biochip journal Vol. 12; no. 2; pp. 123 - 127
• Main Authors: Shin, Soojeong, Kim, Nayeong, Hong, Jong Wook
• Format: Journal Article
• Language: English
• Published: Seoul The Korean BioChip Society (KBCS) 01-06-2018; Springer Nature B.V; 한국바이오칩학회
• Subjects: Adsorption; Biochip products; Biochips; Biocompatibility; Biomedical Engineering and Bioengineering; Biomolecules; Biotechnology; Bovine serum albumin; Chemistry; Chemistry and Materials Science; Contact angle; Controllability; Hydrophobic surfaces; Hydrophobicity; Integrated circuits; Light transmittance; Mineral oils; Original Article; Permeability; Plasma; Polydimethylsiloxane; Polytetrafluoroethylene; Protein adsorption; Proteins; Serum albumin; Spraying; Surface chemistry; 생물공학; Teflon; FITC-BSA; Water-repellent; No plasma treatment; Protein adsorption; FDTS; PDMS; Perfluorodecyltrichlorosilane
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-017-2210-z

Polydimethylsiloxane (PDMS) is commonly used in microchip fabrication due to its biocompatibility, which is essential for biological applications, as well as other properties, including transparency to visible light, controllable gas permeability, mechanical and heat stability, and elasticity. Despite these properties, adsorption of biomolecules remains a major limitation of PDMS in biochips. Methods to prevent sample adsorption have been reported, and herein we compare several surface engineering methods that do not incorporate plasma pretreatment. Three methods - Teflon coating, water-repellent spraying, and perfluorodecyltrichlorosilane (FDTS) blending - were compared by evaluating the amount of fluorescein-isothiocyanate- conjugated bovine serum albumin (FITC-BSA) adsorbed onto the biochips. FDTS-blended PDMS significantly inhibited protein adsorption and showed good oleophobicity, but provided the lowest visible light transmittance of all materials tested.