Contrasting effect of dark-chilling on chloroplast structure and arrangement of chlorophyll-protein complexes in pea and tomato: plants with a different susceptibility to non-freezing temperature

• Published in: Planta Vol. 226; no. 5; pp. 1165 - 1181
• Main Authors: Garstka, Maciej, Venema, Jan Henk, Rumak, Izabela, Gieczewska, Katarzyna, Rosiak, Malgorzata, Koziol-Lipinska, Joanna, Kierdaszuk, Borys, Vredenberg, Wim J, Mostowska, Agnieszka
• Format: Journal Article
• Language: English
• Published: Berlin Berlin/Heidelberg : Springer-Verlag 01-10-2007; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: arabidopsis-thaliana; Biological and medical sciences; Cation-induced thylakoid stacking; Chilling tolerant; Chilling-sensitive; Chlorophyll; Chlorophyll - metabolism; Chlorophyll-protein complexes; Chlorophylls; Chloroplast and thylakoid membrane structure; Chloroplasts; Chloroplasts - ultrastructure; Cold Temperature; cold-acclimation; Confocal laser scanning microscopy; Cooling; cucumber leaves; Dark-chilling stress; Darkness; Electrophoresis, Polyacrylamide Gel; Emission spectra; fatty-acid levels; Fluorescence; Freezing; Fundamental and applied biological sciences. Psychology; Grana; hill reaction activity; Leaves; O-J-D-I-P chlorophyll fluorescence; Peas; Photoactivation; photosynthetic electron-transport; photosystem-i; Physical agents; Pisum sativum - metabolism; Pisum sativum - ultrastructure; Plant physiology and development; Plant Proteins - metabolism; Plants; Solanum lycopersicum - metabolism; Solanum lycopersicum - ultrastructure; soybean genotypes; Spectrometry, Fluorescence; subsequent recovery; thylakoid membranes; Thylakoids; Tomatoes; Vegetative apparatus, growth and morphogenesis. Senescence; Chlorophyll; Temperature; Cold; Chilling-sensitive; Dark-chilling stress Pea; Chlorophyll-protein complexes; Thylakoid; Tomato; Stress; Chloroplast and thylakoid membrane structure; Dicotyledones; Angiospermae; Lycopersicon esculentum; Spermatophyta; Solanaceae; Cation-induced thylakoid stacking O-J-D-I-P chlorophyll fluorescence; Chloroplast; Chilling tolerant; Low temperature; Confocal laser scanning microscopy
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-007-0562-7

The effect of dark-chilling and subsequent photoactivation on chloroplast structure and arrangements of chlorophyll-protein complexes in thylakoid membranes was studied in chilling-tolerant (CT) pea and in chilling-sensitive (CS) tomato. Dark-chilling did not influence chlorophyll content and Chl a/b ratio in thylakoids of both species. A decline of Chl a fluorescence intensity and an increase of the ratio of fluorescence intensities of PSI and PSII at 120 K was observed after dark-chilling in thylakoids isolated from tomato, but not from pea leaves. Chilling of pea leaves induced an increase of the relative contribution of LHCII and PSII fluorescence. A substantial decrease of the LHCII/PSII fluorescence accompanied by an increase of that from LHCI/PSI was observed in thylakoids from chilled tomato leaves; both were attenuated by photoactivation. Chlorophyll fluorescence of bright grana discs in chloroplasts from dark-chilled leaves, detected by confocal laser scanning microscopy, was more condensed in pea but significantly dispersed in tomato, compared with control samples. The chloroplast images from transmission-electron microscopy revealed that dark-chilling induced an increase of the degree of grana stacking only in pea chloroplasts. Analyses of O-J-D-I-P fluorescence induction curves in leaves of CS tomato before and after recovery from chilling indicate changes in electron transport rates at acceptor- and donor side of PS II and an increase in antenna size. In CT pea leaves these effects were absent, except for a small but irreversible effect on PSII activity and antenna size. Thus, the differences in chloroplast structure between CS and CT plants, induced by dark-chilling are a consequence of different thylakoid supercomplexes rearrangements.