Analysis of mutations occurring during replication of a SV40 shuttle vector in mammalian cells

To analyse mutations that arise in mammalian cells we have used a SV40::plasmid shuttle vector containing a portion of the E. coli lacZ gene. We have found that following transfection into monkey Cos-7 cell mutations are not detected in the recovered plasmids at 24 h post transfection, but are found...

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Bibliographic Details
Published in:	Mutation research Vol. 183; no. 3; p. 273
Main Authors:	MacGregor, G R, James, M R, Arlett, C F, Burke, J F
Format:	Journal Article
Language:	English
Published:	Netherlands 01-05-1987
Subjects:	Animals beta-Galactosidase - genetics Cell Line Chromosome Deletion DNA Replication Genetic Vectors Haplorhini Lac Operon Mutation Plasmids Recombination, Genetic Simian virus 40 - genetics Transfection Virus Replication
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Summary:	To analyse mutations that arise in mammalian cells we have used a SV40::plasmid shuttle vector containing a portion of the E. coli lacZ gene. We have found that following transfection into monkey Cos-7 cell mutations are not detected in the recovered plasmids at 24 h post transfection, but are found at 48 h post transfection, after the onset of DNA replication. Analysis of the mutant plasmids shows that in almost all cases the mutant phenotype is caused by a deletion or rearrangement of the lacZ gene in the shuttle vector.
ISSN:	0027-5107
DOI:	10.1016/0167-8817(87)90010-1