Chloroplast-rich material from the physical fractionation of pea vine (Pisum sativum) postharvest field residue (Haulm)

• Published in: Food chemistry Vol. 272; pp. 18 - 25
• Main Authors: Torcello-Gómez, Amelia, Gedi, Mohamed A., Ibbett, Roger, Nawaz Husain, Khatija, Briars, Rhianna, Gray, David
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 30-01-2019
• Subjects: Animal Feed; Animals; Ascorbic Acid - analysis; beta Carotene - analysis; Biomass; Centrifugation; Chlorophyll - analysis; Chloroplast; Chloroplasts - chemistry; Chloroplasts - metabolism; Fatty Acids - analysis; Fatty Acids - chemistry; Micronutrients - chemistry; Micronutrients - isolation & purification; Pea vine; Pisum sativum - chemistry; Pisum sativum - metabolism; α-Linolenic acid; α-Tocopherol; β-Carotene; α-Tocopherol; Pea vine; α-Linolenic acid; Chloroplast; β-Carotene
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2018.08.018

•Chloroplast-rich fraction (CRF) is isolated from pea vine haulm.•Isolation of CRF is done via novel juice extraction by physical fractionation.•CRF from fresh haulm is rich in α-linolenic acid, β-carotene, α-tocopherol.•Wilting and fermenting have great detrimental effect on nutritional content of CRF.•Blanching and freeze storage have lesser effect on nutritional loss of CRF.
An innovative procedure for plant chloroplasts isolation has been proposed, which consists of juice extraction by physical fractionation from plant material and recovery of its chloroplast-rich fraction (CRF) by centrifugation. This simple method has been applied to pea vine haulm subjected to different post-harvest treatments: blanching, storage at different relative humidity values and fermentation. Additionally, freeze storage of the extracted juice was carried out. The macronutrient (total lipids, proteins, ash and carbohydrates) and micronutrient (fatty acids, chlorophylls, β-carotene, α-tocopherol and ascorbic acid) content and composition of the CRF have been determined. The CRF isolated from fresh pea vine haulm is a potential source of essential micronutrients (α-linolenic acid, β-carotene, α-tocopherol) and carbohydrates, whereas the post-harvest treatments trialled have a detrimental effect on the nutritional content. Industrial applications for the recovered nutritionally rich fraction, such as food supplement ingredient or animal feeding, are likely envisaged, while optimising the use of green haulm.