Strategies to increment in vivo and in vitro embryo production and transfer in cattle

• Published in: Animal reproduction Vol. 16; no. 3; pp. 411 - 422
• Main Authors: Bó, Gabriel A, Cedeño, Andrés, Mapletoft, Reuben J
• Format: Journal Article
• Language: English
• Published: Brazil Colégio Brasileiro de Reprodução Animal - CBRA 23-10-2019; Colégio Brasileiro de Reprodução Animal
• Subjects: BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Congress Paper; fixed-time embryo transfer; superstimulation; bovine embryos
• ISSN: 1984-3143; 1806-9614; 1984-3143
• DOI: 10.21451/1984-3143-AR2019-0042

Knowledge of follicular wave dynamics obtained through the use of real-time ultrasonography and the development of the means by which follicular wave dynamics can be controlled have provided practical approaches for the
and
production and transfer of embryos in cattle. The elective control of follicular wave emergence and ovulation has had a great impact on the application of on-farm embryo transfer, especially when large groups of donors need to be superstimulated at the same time. Although estradiol and progestins have been used for many years, practitioners in countries where estradiol cannot be used have turned to alternative treatments, such as mechanical follicle ablation or the administration of GnRH for the synchronization of follicle wave emergence.
embryo production also benefits from the synchronization of follicle wave emergence prior to Cumulus Oocyte Complexes (COCs) recovery. As
cattle have high antral follicle population, large numbers of oocytes can be obtained by ovum pick-up (OPU) without superstimulation. However, synchronization of follicular wave emergence and superstimulation is necessary to obtain high numbers of COCs by OPU and blastocysts following
fertilization in
donors. Finally, embryos can now be transferred in commercial beef or dairy herds using efficacious synchronization and re-synchronization protocols that are easily implemented by farm personnel. These technologies can also be used to resolve reproductive problems such as the reduced fertility observed during summer heat stress and/or in repeat-breeder cows in commercial dairy herds.