A novel adenovirus species associated with an acute respiratory outbreak in a baboon colony and evidence of coincident human infection

• Published in: mBio Vol. 4; no. 2; p. e00084
• Main Authors: Chiu, Charles Y, Yagi, Shigeo, Lu, Xiaoyan, Yu, Guixia, Chen, Eunice C, Liu, Maria, Dick, Jr, Edward J, Carey, Kenneth D, Erdman, Dean D, Leland, Michelle M, Patterson, Jean L
• Format: Journal Article
• Language: English
• Published: United States American Society of Microbiology 16-04-2013; American Society for Microbiology
• Subjects: Adenoviridae Infections - transmission; Adenoviridae Infections - veterinary; Adenoviridae Infections - virology; Adenoviruses, Simian - classification; Adenoviruses, Simian - genetics; Adenoviruses, Simian - isolation & purification; Animals; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; Disease Outbreaks; Humans; Molecular Sequence Data; Occupational Exposure; Papio; Primate Diseases - epidemiology; Primate Diseases - transmission; Primate Diseases - virology; Respiratory Tract Infections - transmission; Respiratory Tract Infections - veterinary; Respiratory Tract Infections - virology; Sequence Analysis, DNA; Texas; Zoonoses - epidemiology; Zoonoses - virology; Texas
• ISSN: 2161-2129; 2150-7511
• DOI: 10.1128/mBio.00084-13

Adenoviruses (AdVs) are DNA viruses that infect many vertebrate hosts, including humans and nonhuman primates. Here we identify a novel AdV species, provisionally named "simian adenovirus C (SAdV-C)," associated with a 1997 outbreak of acute respiratory illness in captive baboons (4 of 9) at a primate research facility in Texas. None of the six AdVs recovered from baboons (BaAdVs) during the outbreak, including the two baboons who died from pneumonia, were typeable. Since clinical samples from the two fatal cases were not available, whole-genome sequencing of nasal isolates from one sick baboon and three asymptomatic baboons during the outbreak was performed. Three AdVs were members of species SAdV-C (BaAdV-2 and BaAdV-4 were genetically identical, and BaAdV-3), while one (BaAdV-1) was a member of the recently described SAdV-B species. BaAdV-3 was the only AdV among the 4 isolated from a sick baboon, and thus was deemed to be the cause of the outbreak. Significant divergence (<58% amino acid identity) was found in one of the fiber proteins of BaAdV-3 relative to BaAdV-2 and -4, suggesting that BaAdV-3 may be a rare SAdV-C recombinant. Neutralizing antibodies to the other 3 AdVs, but not BaAdV-3, were detected in healthy baboons from 1996 to 2003 and staff personnel from 1997. These results implicate a novel adenovirus species (SAdV-C) in an acute respiratory outbreak in a baboon colony and underscore the potential for cross-species transmission of AdVs between humans and nonhuman primates. IMPORTANCE Adenoviruses (AdVs) are DNA viruses that infect many animals, including humans and monkeys. In 1997, an outbreak of acute respiratory illness from AdVs occurred in a baboon colony in Texas. Here we use whole-genome sequencing and antibody testing to investigate new AdVs in baboons (BaAdVs) during the outbreak, one of which, BaAdV-3, came from a sick animal. By sequence analysis, BaAdV-3 may be a recombinant strain that arose from a related BaAdV found in baboons nearby in the colony (who were not sick) and yet another unknown AdV. We also found antibodies to these new BaAdVs in baboons and staff personnel at the facility. Taken together, our findings of a new AdV species as the cause of an acute respiratory outbreak in a baboon colony underscore the ongoing threat from emerging viruses that may carry the potential for cross-species transmission between monkeys and humans.