Moderate hypothermia suppresses jugular venous superoxide anion radical, oxidative stress, early inflammation, and endothelial injury in forebrain ischemia/reperfusion rats

• Published in: Brain research Vol. 1311; pp. 197 - 205
• Main Authors: Koda, Yoichi, Tsuruta, Ryosuke, Fujita, Motoki, Miyauchi, Takashi, Kaneda, Kotaro, Todani, Masaki, Aoki, Tetsuya, Shitara, Masaki, Izumi, Tomonori, Kasaoka, Shunji, Yuasa, Makoto, Maekawa, Tsuyoshi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 22-01-2010; Elsevier
• Subjects: Animals; Anions - metabolism; Biological and medical sciences; Brain - blood supply; Brain - physiopathology; Brain Ischemia - blood; Brain Ischemia - physiopathology; Electrochemical sensor; Encephalitis - blood; Encephalitis - physiopathology; Endothelial Cells - physiology; Endothelium, Vascular - physiopathology; Forebrain ischemia; High-mobility group box 1 (HMGB1) protein; HMGB1 Protein - blood; HMGB1 Protein - metabolism; Hypothermia; Hypothermia - blood; Hypothermia - physiopathology; Hypothermia, Induced; Intercellular Adhesion Molecule-1 - blood; Intercellular Adhesion Molecule-1 - metabolism; Jugular Veins - physiopathology; Male; Malondialdehyde - blood; Malondialdehyde - metabolism; Medical sciences; Neurology; Oxidative Stress - physiology; Random Allocation; Rats; Rats, Wistar; Reperfusion; Reperfusion Injury - blood; Reperfusion Injury - physiopathology; Superoxide anion radical; Time Factors; Vascular diseases and vascular malformations of the nervous system; NADPH; Q R; ICAM-1; HMGB1; MDA; High-mobility group box 1 (HMGB1) protein; sICAM-1; Q; quantified partial value of electricity; SD; SE; Reperfusion; cardiopulmonary arrest; analysis of variance; Q after reperfusion; ANOVA; reactive oxygen species; soluble ICAM-1; MH; malondialdehyde; Electrochemical sensor; xanthine oxidase; moderate hypothermia; intercellular adhesion molecule-1; O 2; forebrain ischemia/reperfusion; PaO 2; partial pressure of oxygen in arterial blood; nicotinamide adenine dinucleotide phosphate; partial pressure of carbon dioxide in arterial blood; FBI/R; standard error; Δ I; superoxide anion radical; high-mobility group box 1; CPA; PaCO 2; ROS; difference in O 2 - · current; mean arterial blood pressure; XO; Q I; Q during ischemia; MAP; Forebrain ischemia; standard deviation; Hypothermia; Superoxide anion radical; Oxidative stress; Rat; Mobility; Cardiovascular disease; Vascular disease; Blood vessel; Cerebrovascular disease; Nervous system diseases; Rodentia; Inflammation; Protein; Cerebral disorder; Endothelium; Vertebrata; Hyperoxides; Mammalia; Animal; Central nervous system disease; Brain ischemia; Early; Circulatory system; Lesion
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2009.11.028

Abstract The aim of this study was to assess the effect of moderate hypothermia (MH) on generation of jugular venous superoxide radical (O2−· ), oxidative stress, early inflammation, and endothelial injury in forebrain ischemia/reperfusion (FBI/R) rats. Twenty-one Wistar rats were allocated to a control group ( n = 7, 37 °C), a pre-MH group ( n = 7, 32 °C before ischemia), and a post-MH group ( n = 7, 32 °C after reperfusion). MH was induced before induction of ischemia in the pre-MH group and just after reperfusion in the post-MH group. Forebrain ischemia was induced by occlusion of bilateral common carotid arteries with hemorrhagic hypotension for 10 min, followed by reperfusion. O2−· in the jugular vein was measured from the produced current using a novel O2−· sensor. The O2−· current showed a gradual increase during forebrain ischemia in the control and post-MH groups but was attenuated in the pre-MH group. Following reperfusion, the current showed a marked increase in the control group but was strongly attenuated in the pre- and post-MH groups. Concentrations of malondialdehyde, high-mobility group box 1 (HMGB1) protein, and intercellular adhesion molecule-1 (ICAM-1) in the brain and plasma 120 min after reperfusion in the pre- and post-MH groups were significantly lower than those in the control group, except for plasma HMGB1 in the post-MH group. In conclusion, MH suppressed O2−· measured in the jugular vein, oxidative stress, early inflammation, and endothelial injury in FBI/R rats.