Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals

While it has been suggested that JC virus (JCV) migrates in B-lymphocytes from the kidney to the central nervous system where it initiates demyelination, this phase of JCV pathogenesis has not been systematically explored. To determine the peripheral blood cell subpopulation(s) infected with JCV, mo...

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Bibliographic Details
Published in:	Journal of neurovirology Vol. 5; no. 4; p. 430
Main Authors:	Koralnik, I J, Schmitz, J E, Lifton, M A, Forman, M A, Letvin, N L
Format:	Journal Article
Language:	English
Published:	United States 01-08-1999
Subjects:	B-Lymphocytes - virology Blotting, Southern DNA, Viral - isolation & purification Flow Cytometry Granulocytes - virology HIV Infections - complications HIV Infections - virology HIV-1 Humans JC Virus - genetics JC Virus - isolation & purification Leukocytes - virology Leukoencephalopathy, Progressive Multifocal - virology Monocytes - virology Polymerase Chain Reaction T-Lymphocytes - virology
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Summary:	While it has been suggested that JC virus (JCV) migrates in B-lymphocytes from the kidney to the central nervous system where it initiates demyelination, this phase of JCV pathogenesis has not been systematically explored. To determine the peripheral blood cell subpopulation(s) infected with JCV, monocytes, granulocytes, and T and B lymphocytes from HIV-1-infected individuals and uninfected controls were purified by flow cytometry. JCV DNA could be detected by PCR amplification in all of these cell subpopulations. This finding suggests that JCV lacks specificity in its interaction with leukocytes.
ISSN:	1355-0284
DOI:	10.3109/13550289909029484