Efficacy of several vaccination programmes in commercial layer and broiler breeder hens against experimental challenge with Salmonella enterica serovar Enteritidis

• Published in: Avian pathology Vol. 38; no. 5; pp. 367 - 375
• Main Authors: Penha Filho, Rafael Antonio Casarin, Paiva, Jacqueline Boldrin de, Arguello, Yuli Melisa Sierra, Silva, Mariana Dias da, Gardin, Yannick, Resende, Fernando, Berchieri Junior, Angelo, Sesti, Luiz
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 01-10-2009; Taylor & Francis Ltd
• Subjects: Animals; bacterial colonization; broiler breeders; Cecum - microbiology; Chickens; Disease Models, Animal; enzyme-linked immunosorbent assay; Experiments; Feces - microbiology; Female; Immunization; laying hens; Liver - microbiology; Poultry; poultry diseases; Poultry Diseases - immunology; Poultry Diseases - microbiology; Poultry Diseases - prevention & control; Salmonella; Salmonella enteritidis; Salmonella enteritidis - immunology; Salmonella Infections, Animal - immunology; Salmonella Infections, Animal - microbiology; Salmonella Infections, Animal - prevention & control; Salmonella Vaccines; seroconversion; serotypes; Spleen - microbiology; vaccination; Vaccination - veterinary; Vaccines, Combined
• ISSN: 0307-9457; 1465-3338
• DOI: 10.1080/03079450903183645

Two experiments were performed to evaluate the protective effect of various vaccination combinations given at 5 and 9 weeks of age against experimental challenge with Salmonella enterica serovar Enteritidis (SE) phage type 4 at 12 weeks of age. In Experiment 1, groups of commercial layers were vaccinated by one of the following programmes: Group 1, two doses of a SE bacterin (Layermune SE); Group 2, one dose of a live Salmonella enterica serovar Gallinarum vaccine (Cevac SG9R) followed by one dose of the SE bacterin; Group 3, one dose of each of two different multivalent inactivated vaccines containing SE cells (Corymune 4K and Corymune 7K; and Group 4, unvaccinated, challenged controls. In Experiment 2, groups of broiler breeders were vaccinated by the same programmes as Groups 1 and 2 above while Group 3 was an unvaccinated, challenged control group. All vaccination programmes and the challenge induced significant (P < 0.05) seroconversion as measured by enzyme-linked immunosorbent assay. Overall, in both experiments, all vaccination schemes were significantly effective in reducing organ (spleen, liver and caeca) colonization by the challenge strain as well as reducing faecal excretion for at least 3 weeks. Vaccinated layers in Groups 1 and 2 and broiler breeders in Group 2 showed the greatest reduction in organ colonization and the least faecal excretion. In Experiment 1, layers vaccinated with multivalent inactivated vaccines containing a SE component (Group 3) were only moderately protected, indicating that such a vaccination programme may be useful in farms with good husbandry and housing conditions and low environmental infectious pressure by Salmonella.