Testing the variability of PSA expression by different human prostate cancer cell lines by means of a new potentiometric device employing molecularly antibody assembled on graphene surface

• Published in: Materials Science & Engineering C Vol. 59; pp. 1069 - 1078
• Main Authors: Rebelo, Tânia S.C.R., Noronha, João P., Galésio, Marco, Santos, Hugo, Diniz, Mário, Sales, M. Goreti F., Fernandes, Maria H., Costa-Rodrigues, João
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-02-2016
• Subjects: Adult; Biological; Biosensing Techniques - instrumentation; Biosensors; Biotechnology; Cancer; Cell Line, Tumor; Cell Proliferation; Culture; Electrochemical biosensor; Equipment Design; Graphene; Graphite - chemistry; Humans; Male; Media; Potentiometry - instrumentation; Prostate; Prostate cell lines; Prostate Specific Antigen; Prostate-Specific Antigen - metabolism; Prostatic Neoplasms - metabolism; Surface Properties; PSCA; KLK4; KLK2; PSMB6; PSMA; Prostate cell lines; Prostate Specific Antigen; PCTA1; PrLZ; Electrochemical biosensor; GUSB; TGM4; PSA
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2015.11.032

Prostate Specific Antigen (PSA) is widely used as a biomarker for prostate cancer. Recently, an electrochemical biosensor for PSA detection by means of molecularly imprinted polymers (MIPs) was developed. This work evaluated the performance and the effectiveness of that PSA biosensor in screening the biomarker PSA in biological media with complex composition, collected from different human prostate cell line cultures. For that, the prostate cancer LNCaP and PC3 cells, and the non-cancerous prostate cell line PNT2 were cultured for 2, 7 and 14days in either α-MEM or RPMI in the presence of 10% or 30% fetal bovine serum. Human gingival fibroblasts were used as a non-cancerous non-prostatic control. The different culture conditions modulated cellular proliferation and the expression of several prostate markers, including PSA. The electrochemical biosensor was able to specifically detect PSA in the culture media and values obtained were similar to those achieved by a commercial Enzyme-Linked Immunosorbent Assay (ELISA) kit, the most commonly used method for PSA quantification in prostate cancer diagnosis. Thus, the tested biosensor may represent a useful alternative as a diagnostic tool for PSA determination in biological samples.
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•PSA quantification was performed in prostate cancer cell culture media.•Culture media composition and culture period significantly affect PSA production.•The PSA biosensor detected a wide range of PSA levels in complex media.•A high data correlation was observed between the biosensor and the ELISA analysis.