Immobilization stress in rat tissues: Alterations in protein oxidation, lipid peroxidation and antioxidant defense system

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology Vol. 144; no. 4; pp. 342 - 347
• Main Authors: Sahin, Emel, Gümüşlü, Saadet
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2007
• Subjects: Animals; Antioxidants - metabolism; Brain - metabolism; Catalase; Catalase - metabolism; Copper,zinc-superoxide dismutase; Corticosterone; Corticosterone - blood; Glutathione - metabolism; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Immobilization stress; Kidney - metabolism; Lipid Peroxidation; Liver - metabolism; Male; Myocardium - metabolism; Protein Carbonylation; Protein oxidation; Rat; Rats; Rats, Wistar; Reduced glutathione; Restraint, Physical; Stomach - metabolism; Stress, Physiological - metabolism; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - metabolism; Tissues; Glutathione peroxidase; Reduced glutathione; Protein oxidation; Catalase; Rat; Corticosterone; Lipid peroxidation; Copper,zinc-superoxide dismutase; Tissues; Immobilization stress
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2006.10.009

We determined the effects of immobilization stress on antioxidant status, protein oxidation and lipid peroxidation in brain, liver, kidney, heart and stomach of rats. Sixteen male Wistar rats (3 months old) were divided into controls (C) and immobilization stress group (IS). IS rats were immobilized for 180 min/day for 15 days. Plasma corticosterone levels were increased in IS group. Copper,zinc-superoxide dismutase activities were increased in brain, liver and kidney, but decreased in the heart and stomach after immobilization. Catalase activities were increased in brain, kidney and heart, and decreased in liver and stomach. Selenium-dependent glutathione peroxidase activities were decreased in brain and kidney, but increased in heart and stomach. Reduced glutathione levels were decreased, while protein carbonyl, conjugated dienes and thiobarbituric acid-reactive substances levels were increased in all tissues. Our results showed that the response of antioxidant defense system to stress differs for each tissue, and protein oxidation and lipid peroxidation is induced by immobilization stress in peripheral tissues.