Post-exercise changes in myostatin and actRIIB expression in obese insulin-resistant rats

• Published in: Molecular and cellular endocrinology Vol. 339; no. 1-2; pp. 159 - 164
• Main Authors: Bueno, P.G., Bassi, D., Contrera, D.G., Carnielli, H.M., Silva, R.N., Nonaka, K.O., Selistre-de-Araújo, H.S., Leal, A.M.O.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 06-06-2011
• Subjects: Activin Receptors, Type II - genetics; Activin Receptors, Type II - metabolism; adipose tissue; Adipose Tissue, Brown - metabolism; Adipose tissues; Adiposity; Animals; Area Under Curve; Body Weight; Dietary Fats; EHF; Endocrinology; energy; Epididymis - pathology; Exercise; Gene Expression; Glucose Tolerance Test; Homeostasis; Insulin Resistance; Intra-Abdominal Fat - metabolism; Intra-Abdominal Fat - pathology; Male; Mesenteric Arteries - pathology; messenger RNA; Muscle, Skeletal - metabolism; Muscles; Myostatin; Myostatin - genetics; Myostatin - metabolism; Obesity; Obesity - metabolism; Physical Conditioning, Animal; Rats; Rats, Wistar; SHF; skeletal muscle; swimming; Obesity; Exercise; MSTN; BAT; TGFβ; Myostatin; ALK5; GAPDH; GDF8; ActRIIB
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/j.mce.2011.04.006

► We evaluated the expression of MSTN and ActRIIB mRNA in different tissues in rats. ► The expression of MSTN and ActRIIB mRNA differed among the three different tissues. ► The MSTN in mesenteric fat tissue was higher in sedentary high-fat diet fed rats. ► In BAT, exercised rats had increased expression of MSTN mRNA. ► In conclusion, we suggest the participation of MSTN in energy homeostasis.
We evaluated the expression of MSTN and ActRIIB mRNA in muscle and adipose tissue in diet-induced obesity and insulin resistance in rats subjected to exercise. There was no difference in the expression of MSTN between exercised and sedentary high-fat fed rats in muscle after swimming training. The expression of ActRIIB mRNA in muscle was not significantly different among the groups. In BAT, MSTN mRNA expression was higher in exercised high-fat fed group (EHF) compared with sedentary high-fat fed group (SHF). ActRIIB mRNA expression in BAT was higher in EHF compared with SHF. In mesenteric fat, MSTN mRNA was lower in EHF compared with SHF and ActRIIB mRNA was lower in EHF compared with SHF. In conclusion, the results demonstrate that the expression of MSTN and ActRIIB mRNA changes in both adipose tissue and skeletal muscle in diet-induced obese and exercised rats and suggest the participation of MSTN in energy homeostasis.