Tff3 -/- Knock-Out Mice with Altered Lipid Metabolism Exhibit a Lower Level of Inflammation following the Dietary Intake of Sodium Chloride for One Week

• Published in: International journal of molecular sciences Vol. 24; no. 8; p. 7315
• Main Authors: Kolobarić, Nikolina, Mihalj, Martina, Kozina, Nataša, Matić, Anita, Mihaljević, Zrinka, Jukić, Ivana, Drenjančević, Ines
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 15-04-2023; MDPI AG
• Subjects: Animals; Blood Pressure; Eating; Inflammation; integrins; knock-out; Lipid Metabolism; Mice; Mice, Knockout; Sodium Chloride; Sodium Chloride, Dietary; trefoil factor-3; trefoil factor-3; integrins; knock-out; inflammation; sodium chloride
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24087315

A high salt intake causes hemodynamic changes and promotes immune response through cell activation and cytokine production, leading to pro-inflammatory conditions. Transgenic
knock-out mice (TFF3ko) (
= 20) and wild-type mice (WT) (
= 20) were each divided into the (1) low-salt (LS) group and (2) high-salt (HS) group. Ten-week-old animals were fed with standard rodent chow (0.4% NaCl) (LS) or food containing 4% NaCl (HS) for one week (7 days). Inflammatory parameters from the sera were measured by Luminex assay. The integrin expression and rates of T cell subsets of interest from the peripheral blood leukocytes (PBLs) and mesenteric lymph nodes (MLNs) were measured using flow cytometry. There was a significant increase in high-sensitivity C reactive protein (hsCRP) only in the WT mice following the HS diet, while there were no significant changes in the serum levels of IFN-γ, TNF-α, IL-2, IL-4, or IL-6 as a response to treatment in either study groups. The rates of CD4
CD25
T cells from MLNs decreased, while CD3
γδTCR
from peripheral blood increased following the HS diet only in TFF3ko. γδTCR expressing T cell rates decreased in WT following the HS diet. The CD49d/VLA-4 expression decreased in the peripheral blood leukocytes in both groups following the HS diet. CD11a/LFA-1 expression significantly increased only in the peripheral blood Ly6C
CD11a
monocytes in WT mice following salt loading. In conclusion, salt-loading in knock-out mice caused a lower level of inflammatory response compared with their control WT mice due to gene depletion.