Viscoelasticity and Volume of Cortical Neurons under Glutamate Excitotoxicity and Osmotic Challenges

• Published in: Biophysical journal Vol. 119; no. 9; pp. 1712 - 1723
• Main Authors: Efremov, Yuri M., Grebenik, Ekaterina A., Sharipov, Rinat R., Krasilnikova, Irina A., Kotova, Svetlana L., Akovantseva, Anastasia A., Bakaeva, Zanda V., Pinelis, Vsevolod G., Surin, Alexander M., Timashev, Peter S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03-11-2020; The Biophysical Society
• Subjects: Animals; Calcium; Cells, Cultured; Cerebral Cortex; Glutamic Acid - toxicity; Neurons; Osmosis; Rats; Viscosity
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/j.bpj.2020.09.022

Neural activity depends on the maintenance of ionic and osmotic homeostasis. Under these conditions, the cell volume must be regulated to maintain optimal neural function. A disturbance in the neuronal volume regulation often occurs in pathological conditions such as glutamate excitotoxicity. The cell volume, mechanical properties, and actin cytoskeleton structure are tightly connected to achieve the cell homeostasis. Here, we studied the effects of glutamate-induced excitotoxicity, external osmotic pressure, and inhibition of actin polymerization on the viscoelastic properties and volume of neurons. Atomic force microscopy was used to map the viscoelastic properties of neurons in time-series experiments to observe the dynamical changes and a possible recovery. The data obtained on cultured rat cortical neurons were compared with the data obtained on rat fibroblasts. The neurons were found to be more responsive to the osmotic challenges but less sensitive to the inhibition of actin polymerization than fibroblasts. The alterations of the viscoelastic properties caused by glutamate excitotoxicity were similar to those induced by the hypoosmotic stress, but, in contrast to the latter, they did not recover after the glutamate removal. These data were consistent with the dynamic volume changes estimated using ratiometric fluorescent dyes. The recovery after the glutamate-induced excitotoxicity was slow or absent because of a steady increase in intracellular calcium and sodium concentrations. The viscoelastic parameters and their changes were related to such parameters as the actin cortex stiffness, tension, and cytoplasmic viscosity.