Effect of the Lambda Repressor on the Binding of RNA Polymerase to DNA

Experiments were designed with the aim of understanding the mechanism whereby the lambda repressor inhibits messenger RNA synthesis. Complexes containing lambda DNA and RNA polymerase were isolated from repressed and derepressed lysogens and analyzed for the relative amounts of RNA polymerase per ph...

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Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 64; no. 3; pp. 962 - 969
Main Authors:	Hayward, William S., Green, Melvin H.
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences of the United States of America 01-11-1969 National Acad Sciences
Subjects:	Bacteriophages Binding Sites Biochemistry Biological Sciences: Biochemistry Cell separation Coliphages - enzymology Coliphages - metabolism DNA DNA, Viral - metabolism Genes, Regulator Genomes Hybridity Hybridization, Genetic Models, Biological Molecules Operator regions Operon Rifampin - pharmacology RNA RNA Nucleotidyltransferases - metabolism RNA, Messenger - biosynthesis Spermatozoa Tritium
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Summary:	Experiments were designed with the aim of understanding the mechanism whereby the lambda repressor inhibits messenger RNA synthesis. Complexes containing lambda DNA and RNA polymerase were isolated from repressed and derepressed lysogens and analyzed for the relative amounts of RNA polymerase per phage genome. The data indicate that the repressor inhibits the binding of RNA polymerase to promoter sites known to be under direct control of the repressor.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Department of Molecular Biology, Albert Einstein College of Medicine, Bronx, New York.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.64.3.962