Biological nitrogen fixation by alternative nitrogenases in terrestrial ecosystems a review

• Published in: Biogeochemistry Vol. 149; no. 1; pp. 53 - 73
• Main Authors: Bellenger, J. P., Darnajoux, R., Zhang, X., Kraepiel, A. M. L.
• Format: Journal Article
• Language: English
• Published: Cham Springer Science + Business Media 01-05-2020; Springer International Publishing; Springer Nature B.V
• Subjects: Ammonium; Ammonium compounds; Bioavailability; Biogeosciences; Boreal ecosystems; Coastal ecosystems; Earth and Environmental Science; Earth Sciences; Ecosystems; Environmental Chemistry; Fresh water; Genes; Iron; Isoforms; Life Sciences; Measurement methods; Molybdenum; Nitrogen; Nitrogen cycle; Nitrogen fixation; Nitrogenase; Nitrogenation; ORIGINAL PAPERS; Soil; Soil bacteria; Soil microorganisms; Symbionts; Symbiosis; Temperature effects; Terrestrial ecosystems; Tropical environments; Vanadium; Iron-only; Alternative nitrogenases; Terrestrial ecosystems; Nitrogenase; Vanadium; Biological nitrogen fixation; Molybdenum
• ISSN: 0168-2563; 1573-515X
• DOI: 10.1007/s10533-020-00666-7

Biological nitrogen fixation (BNF), a key reaction of the nitrogen cycle, is catalyzed by the enzyme nitrogenase. The best studied isoform of this metalloenzyme requires molybdenum (Mo) at its active center to reduce atmospheric dinitrogen (N₂) into bioavailable ammonium. The Mo-dependent nitrogenase is found in all diazotrophs and is the only nitrogenase reported in diazotrophs that form N₂-fixing symbioses with higher plants. In addition to the canonical Mo nitrogenase, two alternative nitrogenases, which use either vanadium (V) or iron (Fe) instead of Mo are known to fix nitrogen. They have been identified in ecologically important groups including free-living bacteria in soils and freshwaters and as symbionts of certain cryptogamic covers. Despite the discovery of these alternative isoforms more than 40 years ago, BNF is still believed to primarily rely on Mo. Here, we review existing studies on alternative nitrogenases in terrestrial settings, spanning inland forests to coastal ecosystems. These studies show frequent Mo limitation of BNF, ubiquitous distribution of alternative nitrogenase genes and significant contributions of alternative nitrogenases to N₂ fixation in ecosystems ranging from the tropics to the subarctic. The effect of temperature on nitrogenase isoform activity and regulation is also discussed. We present recently developed methods for measuring alternative nitrogenase activity in the field and discuss the associated analytical challenges. Finally, we discuss how the enzymatic diversity of nitrogenase forces a re-examination of existing knowledge gaps and our understanding of BNF in nature.