Nicotiana tabacum-associated bioengineered Pseudomonas putida can enhance rhizoremediation of soil containing 2,4-dinitrotoluene

• Published in: 3 Biotech Vol. 10; no. 9; p. 398
• Main Author: Akkaya, Özlem
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-09-2020; Springer Nature B.V
• Subjects: Agriculture; Bacteria; Biodegradation; Bioengineering; Bioinformatics; Biomaterials; Biotechnology; Cancer Research; Chemistry; Chemistry and Materials Science; Degradation; Dinitrotoluene; Inoculum; Nicotiana tabacum; Original; Original Article; Phytoremediation; Pollutants; Pseudomonas putida; Seedlings; Soil contamination; Soil pollution; Soil remediation; Soils; Stem Cells; Tissue culture; Tobacco; Rhizoremediation; 2, 4-DNT degrading bacterium; Plant-rhizobacteria interactions
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-020-02395-y

Rhizoremediation processes are based on plant-bacteria interactions and can be effectively used for cleaning many pollutants from the environment to overcome the constraints of individual phytoremediation. Here, 1 mM and 1.5 mM concentrations of 2,4-dinitrotoluene (2,4-DNT) degrading
Pseudomonas putida
(
P. putida
) strain KT.DNT and various growth stages of
Nicotiana tabacum
(
N. tabacum
) were initially assayed in in vitro tissue culture system and the best conditions for the association of plant-rhizobacterium were ascertained to remediation of the soil contaminated with 2,4-DNT. 5-days old
N. tabacum
plants inoculated with 2 × 10
6
 cfu/mL bacterial inoculum for 3 weeks were preferred for rhizoremediation experiments as they showed a nearly threefold increase in the fresh and dry biomass in comparison to noninoculated ones. When these seedlings were planted either alone or together with
P. putida
KT2440 or
P. putida
KT.DNT in soils contaminated with 1 mM and 1.5 mM of 2,4-DNT, the maximum degradation rate of 98% and ~ 93% were determined at the end of 14 days by KT.DNT inoculated tobacco plants. Our results indicate that it would be advantageous to use the 2,4-DNT-degrading bacterium inoculated with
N. tabacum
plants to accelerate and enhance the cleanup of soil contaminated with 2,4-DNT.