Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers

• Published in: Applied and Environmental Microbiology Vol. 61; no. 2; pp. 817 - 819
• Main Authors: Herman, L.M.F. (Government Dairy Research Station, Melle, Belgium), Block, J.H.G.E. de, Moermans, R.J.B
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-02-1995
• Subjects: ADN; ALIMENTOS; AMPLIFICATION CHAINE POLYMERASE; Animals; Bacteria; Bacteriological methods and techniques used in bacteriology; Bacteriology; Base Sequence; Biochemistry; BIODEGRADACION; BIODEGRADATION; Biological and medical sciences; Colony Count, Microbial; CONTAMINACION; CONTAMINATION; DNA Primers - genetics; DNA, Bacterial - genetics; Evaluation Studies as Topic; Fundamental and applied biological sciences. Psychology; Humans; INFECCION; INFECTION; LAIT; LECHE; LISTERIA MONOCYTOGENES; Listeria monocytogenes - genetics; Listeria monocytogenes - isolation & purification; Microbiology; Milk; Milk - microbiology; Molecular Sequence Data; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - statistics & numerical data; PRODUIT ALIMENTAIRE; REACCION DE CADENAS DE POLIMERASA; Sensitivity and Specificity; Tests; Polymerase chain reaction; Detection limit; Listeria monocytogenes; Bacteria; Method; Serotype; Detection; Milk
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.61.2.817-819.1995

A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes