Blood component resuscitative strategies to mitigate endotheliopathy in a murine hemorrhagic shock model

• Published in: The journal of trauma and acute care surgery Vol. 95; no. 1; pp. 21 - 29
• Main Authors: Baucom, Matthew R., Wallen, Taylor E., Ammann, Allison M., England, Lisa G., Schuster, Rebecca M., Pritts, Timothy A., Goodman, Michael D.
• Format: Journal Article
• Language: English
• Published: United States Lippincott Williams & Wilkins 01-07-2023
• Subjects: Animals; Disease Models, Animal; Isotonic Solutions; Lung Injury; Male; Metalloproteases; Mice; Mice, Inbred C57BL; Resuscitation; Ringer's Lactate; Shock, Hemorrhagic - therapy; Syndecan-1; Ubiquitin Thiolesterase
• ISSN: 2163-0755; 2163-0763; 2163-0763
• DOI: 10.1097/TA.0000000000003942

Resuscitation with plasma components has been shown to improve endotheliopathy induced by hemorrhagic shock, but the optimal resuscitation strategy to preserve the endothelial glycocalyx has yet to be defined. The aim of this study was to determine if resuscitation with lactated Ringer's (LR), whole blood (WB), packed red blood cells (RBCs), platelet-rich plasma (PRP), platelet poor plasma, balanced RBC:PRP (1:1), or day 14 (d14) RBC would best minimize endothelial damage following shock.
Male C57BL/6 mice were hemorrhaged to a goal mean arterial pressure of 25 mm Hg for 1 hour. Unshocked sham mice served as controls. Mice were then resuscitated with equal volumes of LR, WB, RBC, PRP, platelet poor plasma, 1:1, or d14 RBC and then sacrificed at 1, 4, or 24 hours (n = 5). Serum was analyzed for syndecan-1, ubiquitin C-terminal hydrolase L1, and cytokine concentrations. Lungs underwent syndecan-1 immunostaining, and lung injury scores were calculated after hematoxylin and eosin. Proteolytic cleavage of the endothelial glycocalyx was assessed by serum matrix metalloprotease 9 levels.
Serum syndecan-1 and ubiquitin C-terminal hydrolase L1 levels were significantly increased following resuscitation with d14 RBC compared with other groups. Early elevation in lung syndecan-1 staining was noted in LR-treated mice, while d14 mice showed decreased staining compared with sham mice following shock. Lung injury scores were significantly elevated 4 hours after resuscitation with LR and d14 RBC compared with WB. Serum matrix metalloprotease 9 levels were significantly increased at 1 and 4 hours in d14 mice compared with sham mice. Systemic inflammation was increased in animals receiving LR, 1:1, or d14 RBC.
Resuscitation with WB following hemorrhagic shock reduces endothelial syndecan-1 shedding and mitigates lung injury. Aged RBC and LR fail to attenuate endothelial injury following hemorrhagic shock. Further research will be necessary to determine the effect of each of these resuscitative fluids in a hemorrhagic shock model with the addition of tissue injury.