src-related protein tyrosine kinases are physically associated with the surface antigen CD36 in human dermal microvascular endothelial cells

• Published in: FEBS letters Vol. 351; no. 1; pp. 41 - 44
• Main Authors: Bull, Helen A., Brickell, Paul M., Dowd, Pauline M.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 29-08-1994
• Subjects: Antigens, CD - metabolism; BCIP, bromo-4-chloro-3-indoyl phosphate; Blotting, Western; BSA, bovine serum albumin; CD36; CD36 Antigens; Cells, Cultured; DTT, dithiotreitol; ECM, extracellular matrix; Endothelium, Vascular - cytology; Endothelium, Vascular - immunology; HDMEC, human dermal microvascular endothelial cells; Humans; Iscove's DMEM, Iscove's modification of Dulbecco's modified Eagle's medium; LB, lysis buffer; Microvascular endothelium; NBT, nitroblue tetrazolium; NRS, normal rabbit serum; PHS, pooled human serum; PMSF, phenyl methyl sulphonyl fluoride; Protein-Tyrosine Kinases - metabolism; Proto-Oncogene Proteins - metabolism; Proto-Oncogene Proteins c-fyn; Proto-Oncogene Proteins c-yes; Proto-Oncogene Proteins pp60(c-src) - metabolism; PTK, protein tyrosine kinase, HUVEC, human umbilical vein endothelial cells; RIPA, radioimmunoprecipitation assay; SDS, sodium dodecyl sulphate; SDS-PAGE, SDS-polyacrylamide gel electrophoresis; Skin - cytology; Skin - immunology; src-Family Kinases; src-related protein tyrosine kinase; TBS, Tris-buffered saline; SDS-PAGE, SDS-polyacrylamide gel electrophoresis; BSA, bovine serum albumin; NRS, normal rabbit serum; HDMEC, human dermal microvascular endothelial cells; DTT, dithiotreitol; Microvascular endothelium; ECM, extracellular matrix; PTK, protein tyrosine kinase, HUVEC, human umbilical vein endothelial cells; RIPA, radioimmunoprecipitation assay; TBS, Tris-buffered saline; BCIP, bromo-4-chloro-3-indoyl phosphate; src-related protein tyrosine kinase; Iscove's DMEM, Iscove's modification of Dulbecco's modified Eagle's medium; PHS, pooled human serum; LB, lysis buffer; NBT, nitroblue tetrazolium; PMSF, phenyl methyl sulphonyl fluoride; CD36; SDS, sodium dodecyl sulphate
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(94)00814-0

src-related cytoplasmic PTKs are physically and functionally associated with cell surface receptors and are involved in signal transduction. In this paper we report the identification of
src-related proteins p59
fyn
, pp60
c-
scr
and p62
yes
in human microvascular endothelial cells cultured from normal human skin and their physical association with the thrombospondin receptor CD36. Such an association represents a potential signalling pathway by which thrombospondin may regulate angiogenesis.