Synthesis of Kisspeptin-Mimicking Fragments and Investigation of their Skin Anti-Aging Effects

• Published in: International journal of molecular sciences Vol. 21; no. 22; p. 8439
• Main Authors: Lee, Kyung-Eun, Jeong, Sugyeong, Yun, Seok Kyun, Kyung, Seoyeon, Sophie, Abadie, Moh, Sang Hyun, Seo, Hyo Hyun, Park, Myeong Sam, Kang, Seunghyun, Yeo, Hyeonju
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 10-11-2020; MDPI AG
• Subjects: 11-beta-Hydroxysteroid Dehydrogenase Type 1 - antagonists & inhibitors; 11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics; 11β-HSD1; Cell Line; collagen; Collagen Type I - genetics; Collagen Type I - metabolism; Fibroblasts - drug effects; Fibroblasts - metabolism; Gene Expression - drug effects; Humans; Interleukin-6 - genetics; Interleukin-6 - metabolism; Interleukin-8 - genetics; Interleukin-8 - metabolism; Kisspeptin-mimicking fragments; Kisspeptins - chemical synthesis; Kisspeptins - chemistry; Kisspeptins - genetics; Kisspeptins - pharmacology; Kisspeptins - physiology; Matrix Metalloproteinase 1 - genetics; Matrix Metalloproteinase 1 - metabolism; MMP-1; Models, Biological; Models, Molecular; Molecular Mimicry; Molecular Structure; Peptide Fragments - chemical synthesis; Peptide Fragments - chemistry; Peptide Fragments - pharmacology; Protein Conformation; RNA, Messenger - genetics; RNA, Messenger - metabolism; Skin - drug effects; Skin - metabolism; skin aging; Skin Aging - drug effects; Skin Aging - genetics; Skin Aging - physiology; Skin Physiological Phenomena; Solid-Phase Synthesis Techniques; Tissue Culture Techniques; Ultraviolet Rays - adverse effects; collagen; skin aging; Kisspeptin-mimicking fragments; 11β-HSD1; MMP-1
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21228439

In recent years, a number of active materials have been developed to provide anti-aging benefits for skin and, among them, peptides have been considered the most promising candidate due to their remarkable and long-lasting anti-wrinkle activity. Recent studies have begun to elucidate the relationship between the secretion of emotion-related hormones and skin aging. Kisspeptin, a neuropeptide encoded by the
gene, has gained attention in reproductive endocrinology since it stimulates the reproductive axis in the hypothalamus; however, the effects of Kisspeptin on skin have not been studied yet. In this study, we synthesized Kisspeptin-10 and Kisspeptin-E, which are biologically active fragments, to mimic the action of Kisspeptin. Next, we demonstrated the anti-aging effects of the Kisspeptin-mimicking fragments using UV-induced skin aging models, such as UV-induced human dermal fibroblasts (Hs68) and human skin explants. Kisspeptin-E suppressed UV-induced 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) stimulation leading to a regulation of skin aging related genes, including type I procollagen, matrix metalloproteinases-1 (MMP-1), interleukin-6 (IL-6), and IL-8, and rescued the skin integrity. Taken together, these results suggest that Kisspeptin-E could be useful to improve UV-induced skin aging by modulating expression of stress related genes, such as 11β-HSD1.