Transcriptome Responses to Combinations of Stresses in Arabidopsis

• Published in: Plant physiology (Bethesda) Vol. 161; no. 4; pp. 1783 - 1794
• Main Authors: Rasmussen, Simon, Barah, Pankaj, Suarez-Rodriguez, Maria Cristina, Bressendorff, Simon, Friis, Pia, Costantino, Paolo, Bones, Atle M., Nielsen, Henrik Bjørn, Mundy, John
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01-04-2013
• Subjects: Arabidopsis - genetics; Arabidopsis - physiology; Arabidopsis - radiation effects; Biological and medical sciences; Cluster Analysis; Cold Temperature; Combined stress; Connectivity; ECOPHYSIOLOGY AND SUSTAINABILITY; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene expression regulation; Gene Expression Regulation, Plant - radiation effects; Gene Regulatory Networks - genetics; Genes; Light; Metabolism; Molecular Sequence Annotation; Oxidative stress; Pathogens; Plant cells; Plant physiology and development; Plants; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stress response; Stress, Physiological - genetics; Stress, Physiological - radiation effects; Transcriptome - genetics; Transcriptome - radiation effects; Arabidopsis thaliana; Plant physiology; Cruciferae; Dicotyledones; Angiospermae; Transcriptome; Spermatophyta; Experimental plant; Stress
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.112.210773

Biotic and abiotic stresses limit agricultural yields, and plants are often simultaneously exposed to multiple stresses. Combinations of stresses such as heat and drought or cold and high light intensity have profound effects on crop performance and yields. Thus, delineation of the regulatory networks and metabolic pathways responding to single and multiple concurrent stresses is required for breeding and engineering crop stress tolerance. Many studies have described transcriptome changes in response to single stresses. However, exposure of plants to a combination of stress factors may require agonistic or antagonistic responses or responses potentially unrelated to responses to the corresponding single stresses. To analyze such responses, we initially compared transcriptome changes in 10 Arabidopsis (Arabidopsis thaliana) ecotypes using cold, heat, high-light, salt, and flagellin treatments as single stress factors as well as their double combinations. This revealed that some 61% of the transcriptome changes in response to double stresses were not predictable from the responses to single stress treatments. It also showed that plants prioritized between potentially antagonistic responses for only 5% to 10% of the responding transcripts. This indicates that plants have evolved to cope with combinations of stresses and, therefore, may be bred to endure them. In addition, using a subset of this data from the Columbia and Landsberg erecta ecotypes, we have delineated coexpression network modules responding to single and combined stresses.