Electrospun poly(3-hydroxybutyrate-co-4-hydroxybutyrate) /Octacalcium phosphate Nanofibrous membranes for effective guided bone regeneration

• Published in: Materials Science & Engineering C Vol. 112; p. 110763
• Main Authors: Wang, Zetao, Ma, Ke, Jiang, Xianfang, Xie, Jiali, Cai, Peian, Li, Fuxin, Liang, Ruiming, Zhao, Jinmin, Zheng, Li
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-07-2020; Elsevier BV
• Subjects: Animals; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biodegradation; Biomedical materials; Bone and Bones - diagnostic imaging; Bone and Bones - pathology; Bone and Bones - physiology; Bone Density - drug effects; Bone Diseases - therapy; Bone Diseases - veterinary; Bone growth; Bone mineral density; Bone Regeneration - drug effects; Calcium phosphates; Calcium Phosphates - chemistry; Cell Adhesion - drug effects; Cell differentiation; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Computed tomography; Electrospinning; Guided bone regeneration; Hydroxybutyrates - chemistry; Male; Materials science; Mechanical properties; Membranes; Membranes, Artificial; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Mesenchyme; Nanofibers - chemistry; Octacalcium phosphate; Osteoblastogenesis; Osteoblasts; Osteogenesis; Osteogenesis - drug effects; P(3HB-co-4HB); Polyesters - chemistry; Rats; Rats, Sprague-Dawley; Regeneration; Regeneration (physiology); Stem cell transplantation; Stem cells; Stiffness; Tensile strength; Tissue Engineering; γ-Hydroxybutyric acid; Electrospinning; Octacalcium phosphate; Guided bone regeneration; P(3HB-co-4HB); Mesenchymal stem cells
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.110763

Membranes used in guided bone regeneration (GBR) are required to exhibit high mechanical strength, biocompatibility, biodegradation, osteogenic and osteoinductive potential. In our study, poly(3-hydroxybutyrate-co-4-hydroxybutyrate)(P(3HB-co-4HB))/octacalcium phosphate (OCP) (P(3HB-co-4HB)/OCP) nanofibrous membranes were fabricated by electrospinning with two different P(3HB-co-4HB) to OCP ratios (P(3HB-co-4HB):OCP = 95:5 wt% and 90:10 wt%, termed P(3HB-co-4HB)/OCP(5)and P(3HB-co-4HB)/OCP (10), respectively) for GBR. The developed P(3HB-co-4HB)/OCP nanofibrous membranes were analysed for their osteogenic and osteoinductive properties using mesenchymal stem cells (MSCs) in vitro and in a calvarial bone defect rat model. The composite P(3HB-co-4HB)/OCP nanofibrous membranes showed decreased fibre size and enhanced tensile strength compared with those of P(3HB-co-4HB) nanofibrous membranes. In the in vitro studies, the P(3HB-co-4HB)/OCP membranes facilitated cell growth and osteoblastic differentiation of MSCs and were superior to P(3HB-co-4HB) membranes. After covered on the calvarial bone defects, P(3HB-co-4HB)/OCP membranes facilitated greater neobone formation than P(3HB-co-4HB) membranes did, as the result of histological evaluation and micro-CT analysis with higher bone volume/total volume (BV/TV) ratio and bone mineral density (BMD). P(3HB-co-4HB)/OCP(10) membranes with higher OCP content showed greater stiffness and osteoinductivity than P(3HB-co-4HB)/OCP (5)membranes, demonstrating the role of OCP in the composite membranes. These results indicated that electrospun P(3HB-co-4HB)/OCP nanofibrous membranes hold promise for the clinical application of GBR.
•Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)(P(3HB-co-4HB))/octacalcium phosphate (OCP) (P(3HB-co-4HB)/OCP) nanofibrous membranes was fabricated via electrospinning.•The P(3HB-co-4HB)/OCP nanofibrous membranes facilitated cell growth and osteoblastic differentiation of mesenchymal stem cells (MSCs).•The P(3HB-co-4HB)/OCP nanofibrous membrane had superior osteoconductive property and effectively promoted bone formation as regenerative materials for guided bone regeneration.