Cholesterol-linoleic acid liposomes induced extracellular vesicles secretion from immortalized adipose-derived mesenchymal stem cells for in vitro cell migration

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 51; no. 1; pp. 346 - 360
• Main Authors: Chen, Jzit Weii, Liew, Fong Fong, Tan, Hsiao Wei, Misran, Misni, Chung, Ivy
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Ltd 01-12-2023; Taylor & Francis Group
• Subjects: Cell adhesion & migration; Cell interactions; Cell migration; Cholesterol; Extracellular vesicles; immortalized AD-MSCs; Keratinocytes; Light scattering; Linoleic acid; Liposomes; Mesenchymal stem cells; Photon correlation spectroscopy; Physicochemical properties; Regeneration (physiology); regenerative medicine; Secretion; Stem cells; Tissue engineering; Transmission electron microscopy; Vesicles; Wound healing; immortalized AD-MSCs; extracellular vesicles; liposomes; regenerative medicine; Cholesterol
• ISSN: 2169-1401; 2169-141X
• DOI: 10.1080/21691401.2023.2237534

Extracellular vesicles (EVs) are small vesicles that are naturally released by cells and play a crucial role in cell-to-cell communication, tissue repair and regeneration. As naturally secreted EVs are limited, liposomes with different physicochemical properties, such as 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and linoleic acid (LA) with modifications have been formulated to improve EVs secretion for
wound healing. Various analyses, including dynamic light scattering (DLS) and transmission electron microscopy (TEM) were performed to monitor the successful preparation of different types of liposomes. The results showed that cholesterol-LA liposomes significantly improved the secretion of EVs from immortalized adipose-derived mesenchymal stem cells (AD-MSCs) by 1.5-fold. Based on the cell migration effects obtained from scratch assay, both LA liposomal-induced EVs and cholesterol-LA liposomal-induced EVs significantly enhanced the migration of human keratinocytes (HaCaT) cell line. These findings suggested that LA and cholesterol-LA liposomes that enhance EVs secretion are potentially useful and can be extended for various tissue regeneration applications.