Heteroalleles in Common Wheat: Multiple Differences between Allelic Variants of the Gli-B1 Locus

• Published in: International journal of molecular sciences Vol. 22; no. 4; p. 1832
• Main Authors: Metakovsky, Eugene, Pascual, Laura, Vaccino, Patrizia, Melnik, Viktor, Rodriguez-Quijano, Marta, Popovych, Yulia, Chebotar, Sabina, Rogers, William John
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 12-02-2021; MDPI AG
• Subjects: Alleles; APAGE; DNA sequencing; Genes, Plant; Genetic Loci; Gli-B1; Gliadin - genetics; PCR; Pseudogenes; Triticum - genetics; Triticum aestivum; γ-gliadin polymorphism; APAGE; Gli-B1; PCR; Triticum aestivum; DNA sequencing; γ-gliadin polymorphism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22041832

The
-encoded γ-gliadins and non-coding γ-gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major γ-gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers),
-γ-gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene
. It was discovered that encoded γ-gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles
,
,
,
), II (
,
,
), III (
), IV (
,
,
), V (
), VI (
) and VII (
). Allele
(variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the
locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called "heteroallelic". The donor's particularities at the
locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat.