Mutational analysis of the hCDC4 gene in gastric carcinomas

• Published in: European journal of cancer (1990) Vol. 42; no. 14; pp. 2369 - 2373
• Main Authors: Lee, J.W., Soung, Y.H., Kim, H.J., Park, W.S., Nam, S.W., Kim, S.H., Lee, J.Y., Yoo, N.J., Lee, S.H.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-09-2006; Elsevier
• Subjects: Aged; Biological and medical sciences; Cell Cycle Proteins - genetics; Cell Line, Tumor; DNA Mutational Analysis; F-Box Proteins - genetics; F-Box-WD Repeat-Containing Protein 7; Female; Gastric carcinoma; hCDC4; Humans; Male; Medical sciences; Middle Aged; Mutation; Mutation - genetics; Pharmacology. Drug treatments; Stomach Neoplasms - genetics; Tumors; Tumour suppressor gene; Ubiquitin-Protein Ligases - genetics; Tumour suppressor gene; Gastric carcinoma; hCDC4; Mutation; Pharmacology; Malignant tumor; Suppressor gene; Stomach cancer; Stomach carcinoma; Cancerology; Digestive diseases; Genetics; Gastric disease; Tumor suppressor gene
• ISSN: 0959-8049; 1879-0852
• DOI: 10.1016/j.ejca.2005.10.034

hCDC4, a ubiquitin ligase, plays a role in the control of cell cycle and chromosome stability. The hCDC4 gene is considered a tumour suppressor gene and is mutated in several human neoplasias, including colorectal and endometrial tumours. Data on the hCDC4 mutation in gastric cancer is, however, lacking. This study explored the possibility that hCDC4 mutation is involved in the development of gastric cancer. The hCDC4 gene in 162 gastric adenocarcinoma tissues was analysed for somatic mutations using a polymerase chain reaction-single strand conformation polymorphism assay. Overall, six hCDC4 mutations were found (3.7%), comprising four missense, one frameshift deletion and one nonsense mutation(s). It is notable that the hCDC4 mutations were found in early as well as in advanced gastric carcinomas. These data indicate that hCDC4 mutation occasionally occurs in gastric carcinomas and suggest that it might play a role in the development of some gastric carcinomas.