Caerulin-induced pancreatitis in rats： Histological and genetic expression changes from acute phase to recuperation

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 25; pp. 3999 - 4003
• Main Author: Javier Magana-Gómez Guillermo López-Cervantes Ana Maria Calderón de la Barca
• Format: Journal Article
• Language: English
• Published: United States Departamento de Nutrición, Centro de Investigación en Alimentación y Desarrollo, A. C., Hermosillo, Sonora, México%Departamento de Patología,Hospital Infantil del Estado de Sonora, Hermosillo, Sonora,México 07-07-2006; Baishideng Publishing Group Co., Limited
• Subjects: Animals; Antigens, Neoplasm - metabolism; Basic Research; Biomarkers, Tumor - metabolism; Ceruletide; Gene Expression; Lectins, C-Type - metabolism; Male; Pancreas - metabolism; Pancreas - pathology; Pancreatitis - chemically induced; Pancreatitis - metabolism; Pancreatitis - pathology; Pancreatitis-Associated Proteins; Polymerase Chain Reaction; Rats; Rats, Wistar; Recovery of Function - physiology; RNA, Messenger - metabolism; 胰腺炎; 遗传疾病; 雨蛙肽; Acute pancreatitis; Histological changes; L13A mRNA; PAP mRNA
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i25.3999

AIM： To study the histological and pancreatitis-associated protein mRNA accumulation changes of pancreas from acute phase of caerulin-induced pancreatitis to recuperation in rats.
METHODS： Acute pancreatitis was induced by caerulein in male Wistar rats and followed up for 90 d by histological and mRNA analyses of pancreas. Pancreases were dissected at 0, 9, 24 h and 3, 5, 15, 30, 60, 90 d post-induction. Edema （E）, polymorphonuclear neutrophU （pIVlN） infiltration, cytoplasmic vacuolization （V）, zymogen granule depletion （ZD） and acinar disorganization （AD） were microscopically evaluated. Accumulation of pancreatitis- associated protein （PAP） and L13A mRNAs were quantified by real-time PCR.
RESULTS： The main histological changes appeared at 9 h post-induction for PMN infiltration and cytoplasmic V, while at 24 h and 3 d for E and ZD, respectively. All the parameters were recovered after 5 d, except for ZD which delayed more than 30 d. The main AD was observed after 15 d and values returned to normal after 30 d. Similarly to histological changes, accumulation of the PAP mRNA was increased at 9 h with the highest accumulation at 24 h and differences disappeared after 5 d.
CONCLUSION： From the acute phase to recuperation of pancreatitis, regeneration and re-differentiation of pan- creas occur and PAP expression is exclusively an acute response of pancreatitis.