Aged garlic extract protects against methotrexate-induced apoptotic cell injury of IEC-6 cells

Gastrointestinal toxicity is one of the most serious side effects of methotrexate (MTX) treatment. The side effects often disrupt the cancer chemotherapy. We previously reported that aged garlic extract (AGE) protects the small intestine of rats from MTX-induced damage. In this study, the protection...

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Bibliographic Details
Published in:	The Journal of nutrition Vol. 136; no. 3 Suppl; p. 861S
Main Authors:	Horie, Toshiharu, Li, Tiesong, Ito, Kousei, Sumi, Shin-ichiro, Fuwa, Toru
Format:	Journal Article
Language:	English
Published:	United States 01-03-2006
Subjects:	Animals Apoptosis - drug effects Cell Line Cell Survival - drug effects DNA - biosynthesis Garlic Intestinal Mucosa - drug effects Intestinal Mucosa - pathology Kinetics Methotrexate - toxicity Plant Extracts - pharmacology Rats
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Summary:	Gastrointestinal toxicity is one of the most serious side effects of methotrexate (MTX) treatment. The side effects often disrupt the cancer chemotherapy. We previously reported that aged garlic extract (AGE) protects the small intestine of rats from MTX-induced damage. In this study, the protection of AGE against MTX-induced damage of IEC-6 cells originating from the rat jejunum crypt was investigated. MTX decreased the viability of IEC-6 cells, but this effect was prevented by AGE (0.5%). The MTX-induced apoptosis of IEC-6 cells was depressed by AGE. These results indicated that AGE protects IEC-6 cells from the MTX-induced damage. AGE may be useful in cancer chemotherapy with MTX because it reduces MTX-induced intestinal damage.
ISSN:	0022-3166
DOI:	10.1093/jn/136.3.861s