Spontaneously immortalized T lymphocytes from Nijmegen Breakage Syndrome patients display phenotypes typical for lymphoma cells

• Published in: Leukemia research Vol. 32; no. 4; pp. 569 - 577
• Main Authors: Siwicki, Jan Konrad, Rymkiewicz, Grzegorz, Błachnio, Katarzyna, Rygier, Jolanta, Kuźniar, Piotr, Płoski, Rafał, Janusz, Aleksandra, Skurzak, Henryk, Chrzanowska, Krystyna, Steffen, Jan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-04-2008
• Subjects: Anaplastic large cell lymphoma; Cell Line, Transformed - pathology; Cells, Cultured; Flow Cytometry; Gene Rearrangement; Hematology, Oncology and Palliative Medicine; Humans; Immunohistochemistry; Immunophenotyping; In Situ Hybridization, Fluorescence; Lymphoma, Large-Cell, Anaplastic - genetics; Lymphoma, Large-Cell, Anaplastic - pathology; Nijmegen Breakage Syndrome; Nijmegen Breakage Syndrome - pathology; Protein-Tyrosine Kinases - genetics; Receptor Protein-Tyrosine Kinases; Spontaneous immortalization; T-Lymphocytes - pathology; Anaplastic large cell lymphoma; Nijmegen Breakage Syndrome; Spontaneous immortalization
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/j.leukres.2007.08.011

Abstract We found that the peripheral T lymphocytes from four of eight patients with the lymphoma predisposing Nijmegen Breakage Syndrome (NBS) acquired an unlimited growth potential following in vitro mitogen stimulation and subsequent interleukin-2-dependent propagation. The immortal T cell lines revealed morphological and other features typical for anaplastic large cell lymphoma (ALCL). In addition, multiple copies of ALK , but with no ALK gene rearrangements were found in a subpopulation of cells of one of the immortalized lines. These cell lines may be useful for the in vitro elucidation of mechanisms involved in the development of ALCL.