Application of Magnetic Nanoparticles Coated with Crosslinked Zwitterionic Poly(ionic liquid)s for the Extraction of Oligonucleotides

• Published in: Materials Vol. 14; no. 12; p. 3146
• Main Authors: Nuckowski, Łukasz, Dzieszkowski, Krzysztof, Rafiński, Zbigniew, Studzińska, Sylwia
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 08-06-2021; MDPI
• Subjects: Adsorbents; Adsorption; Ammonium acetate; antisense oligonucleotides; Blood plasma; Chromatography; Copolymerization; Dispersion; Divinylbenzene; Extraction procedures; Ion exchange; Ionic liquids; Ions; magnetic dispersive solid-phase extraction; magnetic nanoparticles; Metabolites; Nanoparticles; NMR; Nuclear magnetic resonance; Oligonucleotides; poly(ionic liquid)s; serum samples; Solid phases; Solvents; Zwitterions; United Kingdom > UK; United States > US; Germany
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma14123146

Magnetic nanoparticles coated with zwitterionic poly(ionic liquid)s were applied for dispersive solid-phase extraction of oligonucleotides. The materials were synthesized by miniemulsion copolymerization of ionic liquids and divinylbenzene on magnetic nanoparticles. The functional monomers contain a positively charged imidazolium ring and one of the anionic groups: derivatives of acetate, malonate, or butyl sulfonate ions. Adsorption of unmodified DNA oligonucleotide on obtained materials was possible in ion-exchange (IE) and hydrophilic interactions (HI) mode. The adsorption in IE was possible at low pH and was almost complete. The adsorption in HI mode required the usage of appropriate addition of organic solvent but did not provide full adsorption. Studies on the desorption of the analytes included determining the impact of ammonium acetate concentration and pH and organic solvents addition on the recovery. The material containing acetic fragments as an anionic group was selected for the final procedure with the use of 10 mM ammonium acetate (pH = 9.5)/methanol (50/50, v/v) as an elution solution. The magnetic dispersive solid-phase extraction procedure was tested for the oligonucleotides with various modifications and lengths. Moreover, it was applied to extract DNA oligonucleotide and its synthetic metabolites from enriched human plasma without any pre-purification, with recoveries greater than 80%.