The role of the lecithin addition in the properties and cytotoxic activity of chitosan and chondroitin sulfate nanoparticles containing curcumin

• Published in: Carbohydrate polymers Vol. 227; p. 115351
• Main Authors: Jardim, Katiúscia Vieira, Siqueira, Joseilma Luciana Neves, Báo, Sônia Nair, Sousa, Marcelo Henrique, Parize, Alexandre Luis
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-01-2020
• Subjects: Cell Survival - drug effects; Chitosan; Chitosan - administration & dosage; Chitosan - chemistry; Chondroitin sulfate; Chondroitin Sulfates - administration & dosage; Chondroitin Sulfates - chemistry; Curcumin; Curcumin - administration & dosage; Curcumin - chemistry; Delayed-Action Preparations - administration & dosage; Delayed-Action Preparations - chemistry; Drug delivery; Drug Liberation; Humans; Lecithin; Lecithins - administration & dosage; Lecithins - chemistry; MCF-7 Cells; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Polymeric nanoparticles; Chondroitin sulfate; Curcumin; Drug delivery; Polymeric nanoparticles; Chitosan; Lecithin
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2019.115351

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•CHI/CS and CHI/CS/lecithin NPs were synthesized using the ionic gelation method.•The interaction between biopolymers was optimized by speciation study.•The optimum pH promoted stable suspensions.•CHI/CS/lecithin NPs presented the best characteristics for curcumin encapsulation.•Curcumin loaded in NPs increased the cytotoxic effect against MCF-7 cells.
Surfactants have been used as a tool to improve the properties of polymeric nanoparticles (NPs) and to increase the rate of hydrophobic drug release by means of these nanoparticles. In this context, this study evaluated the effect of lecithin on the characteristics of chitosan (CHI) and chondroitin sulfate (CS) nanoparticles, when applied in curcumin (Curc) release. CHI/CS NPs and CHI/CS/Lecithin NPs were prepared by the ionic gelation method, both as standards and containing curcumin. Simultaneous conductimetric and potentiometric titrations were employed to optimize the interaction between the polymers. NPs with hydrodynamic diameter of ∼130 nm and zeta potential of +60 mV were obtained and characterized by HRTEM; their pore size and surface area were also analyzed by BET method, DLS, FTIR, XPS, and fluorescence spectroscopy techniques to assess morphological and surface properties, stability and interaction between polymers and to quantify the loading of drugs. The final characteristics of NPs were directly influenced by lecithin addition, exhibiting enhanced encapsulation efficiency of curcumin (131.8 μg curcumin per mg CHI/CS/Lecithin/Curc NPs). The release of curcumin occurred gradually through a two-stage process: diffusion-controlled dissolution and release of curcumin controlled by dissolution of the polymer. However, the release of curcumin in buffer solution at pH 7.4 was achieved faster in CHI/CS/Lecithin/Curc NPs than in CHI/CS/Curc NPs. in vitro cytotoxic activity evaluation of the curcumin was determined by the MTT assay, observing that free curcumin and curcumin nanoencapsulated in CHI/CS/Curc and CHI/CS/Lecithin/Curc NPs reduced the viability of MCF-7 cells in the 72 h period (by 28.4, 36.0 and 30.7%, P < 0.0001, respectively). These results indicate that CHI/CS/Lecithin NPs have more appropriate characteristics for encapsulation of curcumin.