Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1

• Published in: The Journal of cell biology Vol. 220; no. 11; p. 1
• Main Authors: Bessy, Thomas, Candelas, Adrian, Souquet, Benoit, Saadallah, Khansa, Schaeffer, Alexandre, Vianay, Benoit, Cuvelier, Damien, Gobaa, Samy, Nakid-Cordero, Cecilia, Lion, Julien, Bories, Jean-Christophe, Mooney, Nuala, Jaffredo, Thierry, Larghero, Jerome, Blanchoin, Laurent, Faivre, Lionel, Brunet, Stephane, Théry, Manuel
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 01-11-2021
• Subjects: Biomedical materials; Bone marrow; Bone Marrow - metabolism; Bone Marrow - physiology; Cell Behavior; Cells, Cultured; Cellular Biology; Chemokine CXCL12 - metabolism; Cytoskeleton; Endothelial cells; Endothelial Cells - metabolism; Endothelial Cells - physiology; Fibroblasts; Hematopoietic stem cells; Hematopoietic Stem Cells - metabolism; Hematopoietic Stem Cells - physiology; Humans; Life Sciences; Mesenchymal Stem Cells - metabolism; Mesenchymal Stem Cells - physiology; Myeloid cells; Polarity; Polarization; Progenitor cells; SDF-1 protein; Stem Cells; Stromal cells
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.202005085

The fate of hematopoietic stem and progenitor cells (HSPCs) is regulated by their interaction with stromal cells in the bone marrow. However, the cellular mechanisms regulating HSPC interaction with these cells and their potential impact on HSPC polarity are still poorly understood. Here we evaluated the impact of cell-cell contacts with osteoblasts or endothelial cells on the polarity of HSPC. We found that an HSPC can form a discrete contact site that leads to the extensive polarization of its cytoskeleton architecture. Notably, the centrosome was located in proximity to the contact site. The capacity of HSPCs to polarize in contact with stromal cells of the bone marrow appeared to be specific, as it was not observed in primary lymphoid or myeloid cells or in HSPCs in contact with skin fibroblasts. The receptors ICAM, VCAM, and SDF1 were identified in the polarizing contact. Only SDF1 was independently capable of inducing the polarization of the centrosome-microtubule network.